Temporally and Spatially Resolved x-ray Fluorescence Measurements of in-situ Drug Concentration in Metered-Dose Inhaler Sprays

Daniel J Duke, Alan L. Kastengren, Nicholas Mason-Smith, Yang Chen, Paul M. Young, Daniela Traini, David A. Lewis, Daniel Edgington-Mitchell, Damon Honnery

Research output: Contribution to journalArticleResearchpeer-review

10 Citations (Scopus)


Purpose: Drug concentration measurements in MDI sprays are typically performed using particle filtration or laser scattering. These techniques are ineffective in proximity to the nozzle, making it difficult to determine how factors such as nozzle design will affect the precipitation of co-solvent droplets in solution-based MDIs, and the final particle distribution. Methods: In optical measurements, scattering from the constituents is difficult to separate. We present a novel technique to directly measure drug distribution. A focused x-ray beam was used to stimulate x-ray fluorescence from the bromine in a solution containing 85% HFA, 15% ethanol co-solvent, and 1 μg / μL IPBr. Results: Instantaneous concentration measurements were obtained with 1 ms temporal resolution and 5 μm spatial resolution, providing information in a region that is inaccessible to many other diagnostics. The drug remains homogeneously mixed over time, but was found to be higher at the centerline than at the periphery. This may have implications for oropharyngeal deposition in vivo. Conclusions: Measurements in the dynamic, turbulent region of MDIs allow us to understand the physical links between formulation, inspiration, and geometry on final particle size and distribution. This will ultimately lead to a better understanding of how MDI design can be improved to enhance respirable fraction.

Original languageEnglish
Pages (from-to)816-825
Number of pages10
JournalPharmaceutical Research
Issue number4
Publication statusPublished - 1 Apr 2016


  • fluorescence
  • pressurized metered dose inhaler
  • synchrotron radiation
  • x-ray

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