TY - JOUR
T1 - Techniques applicable for purifying Chironex fleckeri (box-jellyfish) venom
AU - Othman, Iekhsan
AU - Burnett, Joseph W.
N1 - Funding Information:
Acknowledgements-The investigators were supported in part by a Fullbright Scholarship by the MalaysianAmerican Cultural and Education Exchange and Council for International Exchange of Scholars, Washington, DC (1.0.), The authors acknowledge the aid of: Drs GARY J. CALTON of Rhone-Poulenc Research Center Inc., Savage, MD; JOHN A. WILLIAMSON of Townsville, Australia; and PETER A. FENNER of Mackay, Australia. HELENE RUBINSTEIN and VERONICA SYROMI aided with their technical and secretarial assistance.
PY - 1990
Y1 - 1990
N2 - A survey of several techniques to isolate a purified lethal factor from the tentacles of Chironex fleckeri was completed. Heterologous band patterns were obtained from specific eluates after gel filtration, ion exchange, immunoaffmity and hydrophobic chromatography. SDS-PAGE revealed a dense band at 24,000 mol. wt in many of these fractions. Isoelectric focusing of the crude venom resulted in considerable loss of activity but indicated significant purification in the fractions having a pI of 5.2–6.8. These fractions were also immunologically active against sera from a convalescing post-evenomation patient. The primary difficulties encountered in jellyfish venom purification are the lack of stability and the tendency of the active toxins to adhere to each other and to various support matrices.
AB - A survey of several techniques to isolate a purified lethal factor from the tentacles of Chironex fleckeri was completed. Heterologous band patterns were obtained from specific eluates after gel filtration, ion exchange, immunoaffmity and hydrophobic chromatography. SDS-PAGE revealed a dense band at 24,000 mol. wt in many of these fractions. Isoelectric focusing of the crude venom resulted in considerable loss of activity but indicated significant purification in the fractions having a pI of 5.2–6.8. These fractions were also immunologically active against sera from a convalescing post-evenomation patient. The primary difficulties encountered in jellyfish venom purification are the lack of stability and the tendency of the active toxins to adhere to each other and to various support matrices.
UR - https://www.scopus.com/pages/publications/0025108665
U2 - 10.1016/S0041-0101(09)80005-6
DO - 10.1016/S0041-0101(09)80005-6
M3 - Article
C2 - 1977211
AN - SCOPUS:0025108665
SN - 0041-0101
VL - 28
SP - 821
EP - 835
JO - Toxicon
JF - Toxicon
IS - 7
ER -