Abstract
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
| Original language | English |
|---|---|
| Pages (from-to) | 881-906 |
| Number of pages | 26 |
| Journal | Nanoscale |
| Volume | 10 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 21 Jan 2018 |
| Externally published | Yes |
Cite this
}
Technical challenges of working with extracellular vesicles. / Ramirez, Marcel I.; Amorim, Maria G.; Gadelha, Catarina; Milic, Ivana; Welsh, Joshua A.; Freitas, Vanessa M.; Nawaz, Muhammad; Akbar, Naveed; Couch, Yvonne; Makin, Laura; Cooke, Fiona; Vettore, Andre L.; Batista, Patricia X.; Freezor, Roberta; Pezuk, Julia A.; Rosa-Fernandes, Lívia; Carreira, Ana Claudia O.; Devitt, Andrew; Jacobs, Laura; Silva, Israel T.; Coakley, Gillian; Nunes, Diana N.; Carter, Dave; Palmisano, Giuseppe; Dias-Neto, Emmanuel.
In: Nanoscale, Vol. 10, No. 3, 21.01.2018, p. 881-906.Research output: Contribution to journal › Review Article › Research › peer-review
TY - JOUR
T1 - Technical challenges of working with extracellular vesicles
AU - Ramirez, Marcel I.
AU - Amorim, Maria G.
AU - Gadelha, Catarina
AU - Milic, Ivana
AU - Welsh, Joshua A.
AU - Freitas, Vanessa M.
AU - Nawaz, Muhammad
AU - Akbar, Naveed
AU - Couch, Yvonne
AU - Makin, Laura
AU - Cooke, Fiona
AU - Vettore, Andre L.
AU - Batista, Patricia X.
AU - Freezor, Roberta
AU - Pezuk, Julia A.
AU - Rosa-Fernandes, Lívia
AU - Carreira, Ana Claudia O.
AU - Devitt, Andrew
AU - Jacobs, Laura
AU - Silva, Israel T.
AU - Coakley, Gillian
AU - Nunes, Diana N.
AU - Carter, Dave
AU - Palmisano, Giuseppe
AU - Dias-Neto, Emmanuel
PY - 2018/1/21
Y1 - 2018/1/21
N2 - Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
AB - Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.
UR - http://www.scopus.com/inward/record.url?scp=85040917403&partnerID=8YFLogxK
U2 - 10.1039/c7nr08360b
DO - 10.1039/c7nr08360b
M3 - Review Article
VL - 10
SP - 881
EP - 906
JO - Nanoscale
JF - Nanoscale
SN - 2040-3364
IS - 3
ER -