Technical challenges of working with extracellular vesicles

Marcel I. Ramirez, Maria G. Amorim, Catarina Gadelha, Ivana Milic, Joshua A. Welsh, Vanessa M. Freitas, Muhammad Nawaz, Naveed Akbar, Yvonne Couch, Laura Makin, Fiona Cooke, Andre L. Vettore, Patricia X. Batista, Roberta Freezor, Julia A. Pezuk, Lívia Rosa-Fernandes, Ana Claudia O. Carreira, Andrew Devitt, Laura Jacobs, Israel T. Silva & 5 others Gillian Coakley, Diana N. Nunes, Dave Carter, Giuseppe Palmisano, Emmanuel Dias-Neto

Research output: Contribution to journalReview ArticleResearchpeer-review

Abstract

Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

Original languageEnglish
Pages (from-to)881-906
Number of pages26
JournalNanoscale
Volume10
Issue number3
DOIs
Publication statusPublished - 21 Jan 2018
Externally publishedYes

Cite this

Ramirez, M. I., Amorim, M. G., Gadelha, C., Milic, I., Welsh, J. A., Freitas, V. M., ... Dias-Neto, E. (2018). Technical challenges of working with extracellular vesicles. Nanoscale, 10(3), 881-906. https://doi.org/10.1039/c7nr08360b
Ramirez, Marcel I. ; Amorim, Maria G. ; Gadelha, Catarina ; Milic, Ivana ; Welsh, Joshua A. ; Freitas, Vanessa M. ; Nawaz, Muhammad ; Akbar, Naveed ; Couch, Yvonne ; Makin, Laura ; Cooke, Fiona ; Vettore, Andre L. ; Batista, Patricia X. ; Freezor, Roberta ; Pezuk, Julia A. ; Rosa-Fernandes, Lívia ; Carreira, Ana Claudia O. ; Devitt, Andrew ; Jacobs, Laura ; Silva, Israel T. ; Coakley, Gillian ; Nunes, Diana N. ; Carter, Dave ; Palmisano, Giuseppe ; Dias-Neto, Emmanuel. / Technical challenges of working with extracellular vesicles. In: Nanoscale. 2018 ; Vol. 10, No. 3. pp. 881-906.
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Ramirez, MI, Amorim, MG, Gadelha, C, Milic, I, Welsh, JA, Freitas, VM, Nawaz, M, Akbar, N, Couch, Y, Makin, L, Cooke, F, Vettore, AL, Batista, PX, Freezor, R, Pezuk, JA, Rosa-Fernandes, L, Carreira, ACO, Devitt, A, Jacobs, L, Silva, IT, Coakley, G, Nunes, DN, Carter, D, Palmisano, G & Dias-Neto, E 2018, 'Technical challenges of working with extracellular vesicles' Nanoscale, vol. 10, no. 3, pp. 881-906. https://doi.org/10.1039/c7nr08360b

Technical challenges of working with extracellular vesicles. / Ramirez, Marcel I.; Amorim, Maria G.; Gadelha, Catarina; Milic, Ivana; Welsh, Joshua A.; Freitas, Vanessa M.; Nawaz, Muhammad; Akbar, Naveed; Couch, Yvonne; Makin, Laura; Cooke, Fiona; Vettore, Andre L.; Batista, Patricia X.; Freezor, Roberta; Pezuk, Julia A.; Rosa-Fernandes, Lívia; Carreira, Ana Claudia O.; Devitt, Andrew; Jacobs, Laura; Silva, Israel T.; Coakley, Gillian; Nunes, Diana N.; Carter, Dave; Palmisano, Giuseppe; Dias-Neto, Emmanuel.

In: Nanoscale, Vol. 10, No. 3, 21.01.2018, p. 881-906.

Research output: Contribution to journalReview ArticleResearchpeer-review

TY - JOUR

T1 - Technical challenges of working with extracellular vesicles

AU - Ramirez, Marcel I.

AU - Amorim, Maria G.

AU - Gadelha, Catarina

AU - Milic, Ivana

AU - Welsh, Joshua A.

AU - Freitas, Vanessa M.

AU - Nawaz, Muhammad

AU - Akbar, Naveed

AU - Couch, Yvonne

AU - Makin, Laura

AU - Cooke, Fiona

AU - Vettore, Andre L.

AU - Batista, Patricia X.

AU - Freezor, Roberta

AU - Pezuk, Julia A.

AU - Rosa-Fernandes, Lívia

AU - Carreira, Ana Claudia O.

AU - Devitt, Andrew

AU - Jacobs, Laura

AU - Silva, Israel T.

AU - Coakley, Gillian

AU - Nunes, Diana N.

AU - Carter, Dave

AU - Palmisano, Giuseppe

AU - Dias-Neto, Emmanuel

PY - 2018/1/21

Y1 - 2018/1/21

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AB - Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with potential applications in diagnosis, prognosis and therapeutic guidance in most pathological conditions. These nanosized particles transmit signals determined by their protein, lipid, nucleic acid and sugar content, and the unique molecular pattern of EVs dictates the type of signal to be transmitted to recipient cells. However, their small sizes and the limited quantities that can usually be obtained from patient-derived samples pose a number of challenges to their isolation, study and characterization. These challenges and some possible options to overcome them are discussed in this review.

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U2 - 10.1039/c7nr08360b

DO - 10.1039/c7nr08360b

M3 - Review Article

VL - 10

SP - 881

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JO - Nanoscale

JF - Nanoscale

SN - 2040-3364

IS - 3

ER -

Ramirez MI, Amorim MG, Gadelha C, Milic I, Welsh JA, Freitas VM et al. Technical challenges of working with extracellular vesicles. Nanoscale. 2018 Jan 21;10(3):881-906. https://doi.org/10.1039/c7nr08360b