TCR bias and affinity define two compartments of the CD1b-glycolipid-specific T Cell repertoire
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Current views emphasize TCR diversity as a key feature that differentiates the group 1 (CD1a, CD1b, CD1c) and group 2 (CD1d) CD1 systems. Whereas TCR sequence motifs define CD1d-reactive NKT cells, the available data do not allow a TCR-based organization of the group 1 CD1 repertoire. The observed TCR diversity might result from donor-to-donor differences in TCR repertoire, as seen for MHC-restricted T cells. Alternatively, diversity might result from differing CD1 isoforms, Ags, and methods used to identify TCRs. Using CD1b tetramers to isolate clones recognizing the same glycolipid, we identified a previously unknown pattern of V gene usage (TRAV17, TRBV4-1) among unrelated human subjects. These TCRs are distinct from those present on NKT cells and germline-encoded mycolyl lipid-reactive T cells. Instead, they resemble the TCR of LDN5, one of the first known CD1b-reactive clones that was previously thought to illustrate the diversity of the TCR repertoire. Interdonor TCR conservation was observed in vitro and ex vivo, identifying LDN5-like T cells as a distinct T cell type. These data support TCR-based organization of the CD1b repertoire, which consists of at least two compartments that differ in TCR sequence motifs, affinity, and coreceptor expression.
Van Rhijn, I., Gherardin, N. A., Kasmar, A., de Jager, W., Pellicci, D. G., Kostenko, L., Tan, L. L., Bhati, M., Gras, S., Godfrey, D. I., Rossjohn, J., & Moody, D. B. (2014). TCR bias and affinity define two compartments of the CD1b-glycolipid-specific T Cell repertoire. Journal of Immunology, 192(9), 4054 - 4060. https://doi.org/10.4049/jimmunol.1400158