TCR bias and affinity define two compartments of the CD1b-glycolipid-specific T Cell repertoire

Ildiko Van Rhijn, Nicholas A Gherardin, Anne Kasmar, Wilco de Jager, Daniel G Pellicci, Lyudmila Kostenko, Li Lynn Tan, Mugdha Bhati, Stephanie Gras, Dale I Godfrey, Jamie Rossjohn, D Branch Moody

Research output: Contribution to journalArticleResearchpeer-review

45 Citations (Scopus)

Abstract

Current views emphasize TCR diversity as a key feature that differentiates the group 1 (CD1a, CD1b, CD1c) and group 2 (CD1d) CD1 systems. Whereas TCR sequence motifs define CD1d-reactive NKT cells, the available data do not allow a TCR-based organization of the group 1 CD1 repertoire. The observed TCR diversity might result from donor-to-donor differences in TCR repertoire, as seen for MHC-restricted T cells. Alternatively, diversity might result from differing CD1 isoforms, Ags, and methods used to identify TCRs. Using CD1b tetramers to isolate clones recognizing the same glycolipid, we identified a previously unknown pattern of V gene usage (TRAV17, TRBV4-1) among unrelated human subjects. These TCRs are distinct from those present on NKT cells and germline-encoded mycolyl lipid-reactive T cells. Instead, they resemble the TCR of LDN5, one of the first known CD1b-reactive clones that was previously thought to illustrate the diversity of the TCR repertoire. Interdonor TCR conservation was observed in vitro and ex vivo, identifying LDN5-like T cells as a distinct T cell type. These data support TCR-based organization of the CD1b repertoire, which consists of at least two compartments that differ in TCR sequence motifs, affinity, and coreceptor expression.
Original languageEnglish
Pages (from-to)4054 - 4060
Number of pages7
JournalJournal of Immunology
Volume192
Issue number9
DOIs
Publication statusPublished - 2014

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