@inbook{4ee596fe6f8e49c3ab4921ca053907f3,
title = "Targeting the AAVS1 Site by CRISPR/Cas9 with an Inducible Transgene Cassette for the Neuronal Differentiation of Human Pluripotent Stem Cells",
abstract = "CRISPR/Cas9 system is a powerful genome-editing technology for studying genetics and cell biology. Safe harbor sites are ideal genomic locations for transgene integration with minimal interference in cellular functions. Gene targeting of the AAVS1 locus enables stable transgene expression without phenotypic effects in host cells. Here, we describe the strategy for targeting the AAVS1 site with an inducible Neurogenin-2 (Ngn2) donor template by CRISPR/Cas9 in hiPSCs, which facilitates generation of an inducible cell line that can rapidly and homogenously differentiate into excitatory neurons.",
keywords = "AAVS1, CRISPR/Cas9, Neuronal differentiation, Ngn2, Stem cells",
author = "Jinchao Gu and Ben Rollo and Huseyin Sumer and Brett Cromer",
note = "Funding Information: This work was supported by a Tuition Fee Scholarship from Swinburne University of Technology. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2301-5_6",
language = "English",
isbn = "9781071623008",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "99--114",
editor = "Verma, {Paul John} and Huseyin Sumer and Jun Liu",
booktitle = "Applications of Genome Modulation and Editing",
address = "United States of America",
}