Targeting of tail-anchored proteins to yeast mitochondria in vivo

Billie Egan, Traude Beilharz, Rebecca George, Sandra Isenmann, Sabine Gratzer, Binks Wattenberg, Trevor Lithgow

Research output: Contribution to journalArticleResearchpeer-review

65 Citations (Scopus)

Abstract

Tail-anchored proteins are inserted into intracellular membranes via a C-terminal transmembrane domain. The topology of the protein is such that insertion must occur post-translationally, since the insertion sequence is not available for membrane insertion until after translation of the tail-anchored polypeptide is completed. Here, we show that the targeting information in one such tail-anchored protein, translocase in the outer mitochondrial membrane 22, is contained in a short region flanking the transmembrane domain. An equivalent region is sufficient to specify the localisation of Bcl2 and SNARE proteins to the secretory membranes. We discuss the targeting process for directing members of this protein family to the secretory and mitochondrial membranes in vivo. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)243-248
Number of pages6
JournalFEBS Letters
Volume451
Issue number3
DOIs
Publication statusPublished - 28 May 1999
Externally publishedYes

Keywords

  • Membrane protein
  • Mitochondrion
  • Protein translocation
  • Tail anchor
  • Transmembrane domain

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