Targeting of a natural killer cell receptor family by a viral immunoevasin

Richard Berry, Natasha May-Yoke Ng, Philippa M Saunders, Julian Vivian, Jie Lin, Felix Deuss, Alexandra Corbett, Catherine A Forbes, Jacqueline ML Widjaja, Lucy Catherine Sullivan, Adrian Dale McAlister, Matthew Anthony Perugini, Melissa J Call, Anthony A Scalzo, Mariapia Degli-Esposti, Jerome D Coudert, Travis Clarke Beddoe, Andrew Brooks, Jamie Rossjohn

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

Activating and inhibitory receptors on natural killer (NK) cells have a crucial role in innate immunity, although the basis of the engagement of activating NK cell receptors is unclear. The activating receptor Ly49H confers resistance to infection with murine cytomegalovirus by binding to the immunoevasin m157. We found that m157 bound to the helical stalk of Ly49H, whereby two m157 monomers engaged the Ly49H dimer. The helical stalks of Ly49H lay centrally across the m157 platform, whereas its lectin domain was not required for recognition. Instead, m157 targeted an aromatic peg motif present in stalks of both activating and inhibitory receptors of the Ly49 family, and substitution of this motif abrogated binding. Furthermore, ligation of m157 to Ly49H or Ly49C resulted in intracellular signaling. Accordingly, m157 has evolved to tackle the legs of a family of NK cell receptors.
Original languageEnglish
Pages (from-to)699 - 707
Number of pages9
JournalNature Immunology
Volume14
Issue number7
DOIs
Publication statusPublished - 2013

Cite this

Berry, Richard ; Ng, Natasha May-Yoke ; Saunders, Philippa M ; Vivian, Julian ; Lin, Jie ; Deuss, Felix ; Corbett, Alexandra ; Forbes, Catherine A ; Widjaja, Jacqueline ML ; Sullivan, Lucy Catherine ; McAlister, Adrian Dale ; Perugini, Matthew Anthony ; Call, Melissa J ; Scalzo, Anthony A ; Degli-Esposti, Mariapia ; Coudert, Jerome D ; Beddoe, Travis Clarke ; Brooks, Andrew ; Rossjohn, Jamie. / Targeting of a natural killer cell receptor family by a viral immunoevasin. In: Nature Immunology. 2013 ; Vol. 14, No. 7. pp. 699 - 707.
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abstract = "Activating and inhibitory receptors on natural killer (NK) cells have a crucial role in innate immunity, although the basis of the engagement of activating NK cell receptors is unclear. The activating receptor Ly49H confers resistance to infection with murine cytomegalovirus by binding to the immunoevasin m157. We found that m157 bound to the helical stalk of Ly49H, whereby two m157 monomers engaged the Ly49H dimer. The helical stalks of Ly49H lay centrally across the m157 platform, whereas its lectin domain was not required for recognition. Instead, m157 targeted an aromatic peg motif present in stalks of both activating and inhibitory receptors of the Ly49 family, and substitution of this motif abrogated binding. Furthermore, ligation of m157 to Ly49H or Ly49C resulted in intracellular signaling. Accordingly, m157 has evolved to tackle the legs of a family of NK cell receptors.",
author = "Richard Berry and Ng, {Natasha May-Yoke} and Saunders, {Philippa M} and Julian Vivian and Jie Lin and Felix Deuss and Alexandra Corbett and Forbes, {Catherine A} and Widjaja, {Jacqueline ML} and Sullivan, {Lucy Catherine} and McAlister, {Adrian Dale} and Perugini, {Matthew Anthony} and Call, {Melissa J} and Scalzo, {Anthony A} and Mariapia Degli-Esposti and Coudert, {Jerome D} and Beddoe, {Travis Clarke} and Andrew Brooks and Jamie Rossjohn",
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pages = "699 -- 707",
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Berry, R, Ng, NM-Y, Saunders, PM, Vivian, J, Lin, J, Deuss, F, Corbett, A, Forbes, CA, Widjaja, JML, Sullivan, LC, McAlister, AD, Perugini, MA, Call, MJ, Scalzo, AA, Degli-Esposti, M, Coudert, JD, Beddoe, TC, Brooks, A & Rossjohn, J 2013, 'Targeting of a natural killer cell receptor family by a viral immunoevasin', Nature Immunology, vol. 14, no. 7, pp. 699 - 707. https://doi.org/10.1038/ni.2605

Targeting of a natural killer cell receptor family by a viral immunoevasin. / Berry, Richard; Ng, Natasha May-Yoke; Saunders, Philippa M; Vivian, Julian; Lin, Jie; Deuss, Felix; Corbett, Alexandra; Forbes, Catherine A; Widjaja, Jacqueline ML; Sullivan, Lucy Catherine; McAlister, Adrian Dale; Perugini, Matthew Anthony; Call, Melissa J; Scalzo, Anthony A; Degli-Esposti, Mariapia; Coudert, Jerome D; Beddoe, Travis Clarke; Brooks, Andrew; Rossjohn, Jamie.

In: Nature Immunology, Vol. 14, No. 7, 2013, p. 699 - 707.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Targeting of a natural killer cell receptor family by a viral immunoevasin

AU - Berry, Richard

AU - Ng, Natasha May-Yoke

AU - Saunders, Philippa M

AU - Vivian, Julian

AU - Lin, Jie

AU - Deuss, Felix

AU - Corbett, Alexandra

AU - Forbes, Catherine A

AU - Widjaja, Jacqueline ML

AU - Sullivan, Lucy Catherine

AU - McAlister, Adrian Dale

AU - Perugini, Matthew Anthony

AU - Call, Melissa J

AU - Scalzo, Anthony A

AU - Degli-Esposti, Mariapia

AU - Coudert, Jerome D

AU - Beddoe, Travis Clarke

AU - Brooks, Andrew

AU - Rossjohn, Jamie

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AB - Activating and inhibitory receptors on natural killer (NK) cells have a crucial role in innate immunity, although the basis of the engagement of activating NK cell receptors is unclear. The activating receptor Ly49H confers resistance to infection with murine cytomegalovirus by binding to the immunoevasin m157. We found that m157 bound to the helical stalk of Ly49H, whereby two m157 monomers engaged the Ly49H dimer. The helical stalks of Ly49H lay centrally across the m157 platform, whereas its lectin domain was not required for recognition. Instead, m157 targeted an aromatic peg motif present in stalks of both activating and inhibitory receptors of the Ly49 family, and substitution of this motif abrogated binding. Furthermore, ligation of m157 to Ly49H or Ly49C resulted in intracellular signaling. Accordingly, m157 has evolved to tackle the legs of a family of NK cell receptors.

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JO - Nature Immunology

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SN - 1529-2908

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