Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation

Jessica M Moffat, Elodie Segura, Gabriela Khoury, Irina Caminschi, Paul Urquhart Cameron, Sharon R Lewin, Jose A Villadangos, Justine Mintern

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Abstract

Bone marrow stromal cell-2 (BST-2) has major roles in viral tethering and modulation of interferon production. Here we investigate BST-2 as a receptor for the delivery of antigen to dendritic cells (DCs). We show that BST-2 is expressed by a panel of mouse and human DC subsets, particularly under inflammatory conditions. The outcome of delivering antigen to BST-2 expressed by steady state and activated plasmacytoid DC (pDC) or conventional CD8+ and CD8- DCs was determined. T-cell responses were measured for both MHC class I (MHCI) and MHC class II (MHCII) antigen presentation pathways in vitro. Delivering antigen via BST-2 was compared with that via receptors DEC205 or Siglec-H. We show that despite a higher antigen load and faster receptor internalisation, when antigen is delivered to steady state or activated pDC via BST-2, BST-2-targeted activated conventional DCs present antigen more efficiently. Relative to DEC205, BST-2 was inferior in its capacity to deliver antigen to the MHCI cross-presentation pathway. In contrast, BST-2 was superior to Siglec-H at initiating either MHCI or MHCII antigen presentation. In summary, BST-2 is a useful receptor to target with antigen, given its broad expression pattern and ability to access both MHCI and MHCII presentation pathways with relative efficiency.
Original languageEnglish
Pages (from-to)595 - 605
Number of pages11
JournalEuropean Journal of Immunology
Volume43
Issue number3
DOIs
Publication statusPublished - 2013

Cite this

Moffat, Jessica M ; Segura, Elodie ; Khoury, Gabriela ; Caminschi, Irina ; Cameron, Paul Urquhart ; Lewin, Sharon R ; Villadangos, Jose A ; Mintern, Justine. / Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation. In: European Journal of Immunology. 2013 ; Vol. 43, No. 3. pp. 595 - 605.
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abstract = "Bone marrow stromal cell-2 (BST-2) has major roles in viral tethering and modulation of interferon production. Here we investigate BST-2 as a receptor for the delivery of antigen to dendritic cells (DCs). We show that BST-2 is expressed by a panel of mouse and human DC subsets, particularly under inflammatory conditions. The outcome of delivering antigen to BST-2 expressed by steady state and activated plasmacytoid DC (pDC) or conventional CD8+ and CD8- DCs was determined. T-cell responses were measured for both MHC class I (MHCI) and MHC class II (MHCII) antigen presentation pathways in vitro. Delivering antigen via BST-2 was compared with that via receptors DEC205 or Siglec-H. We show that despite a higher antigen load and faster receptor internalisation, when antigen is delivered to steady state or activated pDC via BST-2, BST-2-targeted activated conventional DCs present antigen more efficiently. Relative to DEC205, BST-2 was inferior in its capacity to deliver antigen to the MHCI cross-presentation pathway. In contrast, BST-2 was superior to Siglec-H at initiating either MHCI or MHCII antigen presentation. In summary, BST-2 is a useful receptor to target with antigen, given its broad expression pattern and ability to access both MHCI and MHCII presentation pathways with relative efficiency.",
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Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation. / Moffat, Jessica M; Segura, Elodie; Khoury, Gabriela; Caminschi, Irina; Cameron, Paul Urquhart; Lewin, Sharon R; Villadangos, Jose A; Mintern, Justine.

In: European Journal of Immunology, Vol. 43, No. 3, 2013, p. 595 - 605.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Moffat, Jessica M

AU - Segura, Elodie

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AU - Cameron, Paul Urquhart

AU - Lewin, Sharon R

AU - Villadangos, Jose A

AU - Mintern, Justine

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