Targeting and uptake of multilayered particles to colorectal cancer cells

The targeting and uptake of multilayered particles to colorectal cancer cells are discussed. The polymer capsules formed by the layer-by-layer (LbL) assembly technique have the potential to perform the dual role of protecting the body from harmful side effects of the therapeutic and protecting the therapeutic from degradation by the body. A strategy is followed to achieve targeting, utilizes antibodies, that bind to tumor-associated markers on cells in immunotherapeutic approaches to cancer treatment. Humanized A33 monoclonal antibody binds to a transmembrane human A33 antigen known as glycoprotein and this binding promotes rapid internalization of the antibody with the triggering of particles. The LbL particles biofunctionalized with huA33 mAb have the potential for targeting colorectal cells with high loading, retained stability of the drug, and considerable reduction of unwanted effects to surrounding tissues or cells.