Targeted mutagenesis: Analysis of phenotype without germ line transmission

A. Nagy, J. Rossant

Research output: Contribution to journalReview ArticleResearchpeer-review

32 Citations (Scopus)

Abstract

The available techniques for directed gene manipulation in the mouse are unprecedented in any multicellular organism and make the mouse an invaluable tool for unraveling all aspects of mammalian biology. To realize fully the potential of these genetic tools requires that phenotypic analysis be efficient, rapid, and complete. Genetic chimeras and mosaics, in which mutant cells are mixed with wild-type cells, can be used to augment standard analysis of intact mutant animals and alleviate the time required and the expense involved in generating and maintaining multiple strains of mutant mice.

Original languageEnglish
Pages (from-to)1360-1365
Number of pages6
JournalJournal of Clinical Investigation
Volume97
Issue number6
DOIs
Publication statusPublished - 15 Mar 1996
Externally publishedYes

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