Targeted antithrombotic protein micelles

Wookhyun Kim, Carolyn A Haller, Erbin Dai, Xiaowei Wang, Christoph Eugen Hagemeyer, David Ruchien Liu, Karlheinz Peter, Elliot L Chaikof

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

Activated platelets provide a promising target for imaging inflammatory and thrombotic events along with site-specific delivery of a variety of therapeutic agents. Multifunctional protein micelles bearing targeting and therapeutic proteins were now obtained by one-pot transpeptidation using an evolved sortase A. Conjugation to the corona of a single-chain antibody (scFv), which binds to the ligand-induced binding site (LIBS) of activated GPIIb/IIIa receptors, enabled the efficient detection of thrombi. The inhibition of thrombus formation was subsequently accomplished by incorporating the catalytically active domain of thrombomodulin (TM) onto the micelle corona for the local generation of activated protein C, which inhibits the formation of thrombin. An effective strategy has been developed for the preparation of protein micelles that can be targeted to sites of activated platelets with broad potential for treatment of acute thrombotic events.
Original languageEnglish
Pages (from-to)1461 - 1465
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number5
DOIs
Publication statusPublished - 2015
Externally publishedYes

Cite this

Kim, Wookhyun ; Haller, Carolyn A ; Dai, Erbin ; Wang, Xiaowei ; Hagemeyer, Christoph Eugen ; Liu, David Ruchien ; Peter, Karlheinz ; Chaikof, Elliot L. / Targeted antithrombotic protein micelles. In: Angewandte Chemie - International Edition. 2015 ; Vol. 54, No. 5. pp. 1461 - 1465.
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abstract = "Activated platelets provide a promising target for imaging inflammatory and thrombotic events along with site-specific delivery of a variety of therapeutic agents. Multifunctional protein micelles bearing targeting and therapeutic proteins were now obtained by one-pot transpeptidation using an evolved sortase A. Conjugation to the corona of a single-chain antibody (scFv), which binds to the ligand-induced binding site (LIBS) of activated GPIIb/IIIa receptors, enabled the efficient detection of thrombi. The inhibition of thrombus formation was subsequently accomplished by incorporating the catalytically active domain of thrombomodulin (TM) onto the micelle corona for the local generation of activated protein C, which inhibits the formation of thrombin. An effective strategy has been developed for the preparation of protein micelles that can be targeted to sites of activated platelets with broad potential for treatment of acute thrombotic events.",
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Targeted antithrombotic protein micelles. / Kim, Wookhyun; Haller, Carolyn A; Dai, Erbin; Wang, Xiaowei; Hagemeyer, Christoph Eugen; Liu, David Ruchien; Peter, Karlheinz; Chaikof, Elliot L.

In: Angewandte Chemie - International Edition, Vol. 54, No. 5, 2015, p. 1461 - 1465.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Kim, Wookhyun

AU - Haller, Carolyn A

AU - Dai, Erbin

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AU - Hagemeyer, Christoph Eugen

AU - Liu, David Ruchien

AU - Peter, Karlheinz

AU - Chaikof, Elliot L

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AB - Activated platelets provide a promising target for imaging inflammatory and thrombotic events along with site-specific delivery of a variety of therapeutic agents. Multifunctional protein micelles bearing targeting and therapeutic proteins were now obtained by one-pot transpeptidation using an evolved sortase A. Conjugation to the corona of a single-chain antibody (scFv), which binds to the ligand-induced binding site (LIBS) of activated GPIIb/IIIa receptors, enabled the efficient detection of thrombi. The inhibition of thrombus formation was subsequently accomplished by incorporating the catalytically active domain of thrombomodulin (TM) onto the micelle corona for the local generation of activated protein C, which inhibits the formation of thrombin. An effective strategy has been developed for the preparation of protein micelles that can be targeted to sites of activated platelets with broad potential for treatment of acute thrombotic events.

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