Tandem stenosis to induce atherosclerotic plaque instability in the mouse

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Despite the number of animal models of atherosclerosis, a major limitation in research on mechanisms of plaque rupture is the lack of appropriate atherosclerotic mouse models where lesions develop and progress to a vulnerable and thus rupture-prone phenotype that is typically observed in humans. Most animal models of atherosclerosis typically represent a few but not the full combination of the characteristics seen in human unstable/ruptured plaques. Such characteristics most importantly include a thin and ruptured fibrous cap, plaque inflammation, neovascularization within the plaque (vasa vasorum), plaque hemorrhage, and intravascular (often occlusive) thrombus formation. Ideally, an animal model of plaque instability/rupture would respond to current pharmacological interventions known to reduce the risk of plaque rupture, such as statins. Here we describe a mouse model of plaque instability/rupture that is based on the surgical introduction of a tandem stenosis in the carotid artery. This model results in the formation of unstable atherosclerotic plaques that reflect human plaque pathology. It will allow to further understanding of plaque instability/rupture, to identify the participating factors such as specific proteins, genes and microRNAs, and to develop imaging methods towards the detection of vulnerable, rupture-prone atherosclerotic plaques

Original languageEnglish
Title of host publicationMethods in Mouse Atherosclerosis
EditorsVicente Andres, Beatriz Dorado
Place of PublicationNew York NY USA
PublisherHumana Press
Number of pages6
ISBN (Electronic)9781493929290
ISBN (Print)9781493929283
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Acute myocardial infarction
  • Angiogenesis animal models of human disease
  • Arterial thrombosis
  • Atherosclerosis
  • Gene expression profiling
  • Inflammation
  • MicroRNA profiling
  • Plaque rupture

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