Tandem stenosis to induce atherosclerotic plaque instability in the mouse

Yung Chih Chen, Jennifer Rivera, Karlheinz Peter

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

4 Citations (Scopus)

Abstract

Despite the number of animal models of atherosclerosis, a major limitation in research on mechanisms of plaque rupture is the lack of appropriate atherosclerotic mouse models where lesions develop and progress to a vulnerable and thus rupture-prone phenotype that is typically observed in humans. Most animal models of atherosclerosis typically represent a few but not the full combination of the characteristics seen in human unstable/ruptured plaques. Such characteristics most importantly include a thin and ruptured fibrous cap, plaque inflammation, neovascularization within the plaque (vasa vasorum), plaque hemorrhage, and intravascular (often occlusive) thrombus formation. Ideally, an animal model of plaque instability/rupture would respond to current pharmacological interventions known to reduce the risk of plaque rupture, such as statins. Here we describe a mouse model of plaque instability/rupture that is based on the surgical introduction of a tandem stenosis in the carotid artery. This model results in the formation of unstable atherosclerotic plaques that reflect human plaque pathology. It will allow to further understanding of plaque instability/rupture, to identify the participating factors such as specific proteins, genes and microRNAs, and to develop imaging methods towards the detection of vulnerable, rupture-prone atherosclerotic plaques

Original languageEnglish
Title of host publicationMethods in Molecular Biology
EditorsVicente Andres, Beatriz Dorado
Place of PublicationNew York NY USA
PublisherHumana Press
Chapter23
Pages333-338
Number of pages6
Volume1339
ISBN (Electronic)9781493929290
ISBN (Print)9781493929283
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
Volume1339
ISSN (Print)1064-3745

Keywords

  • Acute myocardial infarction
  • Angiogenesis animal models of human disease
  • Arterial thrombosis
  • Atherosclerosis
  • Gene expression profiling
  • Inflammation
  • MicroRNA profiling
  • Plaque rupture

Cite this

Chen, Y. C., Rivera, J., & Peter, K. (2015). Tandem stenosis to induce atherosclerotic plaque instability in the mouse. In V. Andres, & B. Dorado (Eds.), Methods in Molecular Biology (Vol. 1339, pp. 333-338). (Methods in Molecular Biology; Vol. 1339). New York NY USA: Humana Press. https://doi.org/10.1007/978-1-4939-2929-0_23
Chen, Yung Chih ; Rivera, Jennifer ; Peter, Karlheinz. / Tandem stenosis to induce atherosclerotic plaque instability in the mouse. Methods in Molecular Biology. editor / Vicente Andres ; Beatriz Dorado. Vol. 1339 New York NY USA : Humana Press, 2015. pp. 333-338 (Methods in Molecular Biology).
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Chen, YC, Rivera, J & Peter, K 2015, Tandem stenosis to induce atherosclerotic plaque instability in the mouse. in V Andres & B Dorado (eds), Methods in Molecular Biology. vol. 1339, Methods in Molecular Biology, vol. 1339, Humana Press, New York NY USA, pp. 333-338. https://doi.org/10.1007/978-1-4939-2929-0_23

Tandem stenosis to induce atherosclerotic plaque instability in the mouse. / Chen, Yung Chih; Rivera, Jennifer; Peter, Karlheinz.

Methods in Molecular Biology. ed. / Vicente Andres; Beatriz Dorado. Vol. 1339 New York NY USA : Humana Press, 2015. p. 333-338 (Methods in Molecular Biology; Vol. 1339).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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Chen YC, Rivera J, Peter K. Tandem stenosis to induce atherosclerotic plaque instability in the mouse. In Andres V, Dorado B, editors, Methods in Molecular Biology. Vol. 1339. New York NY USA: Humana Press. 2015. p. 333-338. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-2929-0_23