TY - JOUR
T1 - Tampons
T2 - A novel patient-administered method for the assessment of genital human papillomavirus infection
AU - Fairley, Christopher K.
AU - Chen, Shujun
AU - Tabrizi, Sepehr N.
AU - Quinn, Michael A.
AU - McNeil, John J.
AU - Garland, Suzanne M.
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Assessment of human papillomavirus (HPV) infection usually requires a speculum examination to collect genital specimens. A technique using tampons as a patient-administered method for the collection of specimens was studied by dot blot hybridization (HPV types 6, 11,16,18, 31, and 33) and polymerase chain reaction (PCR). Tampons and cervical scrapes were collected from 48 consecutive women attending a dysplasia clinic. Tampons provided a significantly larger pellet volume (P <.002) and more DNA (P <.01) than scrapes. There was a close correlation when samples were analyzed for the presence of HPV DNA. Using dot blot hybridization, 8 cervical scrapes (17%) and 9 tampons (19%) were positive for HPV DNA (90% correlation). By PCR, 35 cervical scrapes (73%) and 33 tampons (69%) were positive for HPV DNA (88% correlation). Thus, tampon specimens are an easy method for assessment of genital HPV infection.
AB - Assessment of human papillomavirus (HPV) infection usually requires a speculum examination to collect genital specimens. A technique using tampons as a patient-administered method for the collection of specimens was studied by dot blot hybridization (HPV types 6, 11,16,18, 31, and 33) and polymerase chain reaction (PCR). Tampons and cervical scrapes were collected from 48 consecutive women attending a dysplasia clinic. Tampons provided a significantly larger pellet volume (P <.002) and more DNA (P <.01) than scrapes. There was a close correlation when samples were analyzed for the presence of HPV DNA. Using dot blot hybridization, 8 cervical scrapes (17%) and 9 tampons (19%) were positive for HPV DNA (90% correlation). By PCR, 35 cervical scrapes (73%) and 33 tampons (69%) were positive for HPV DNA (88% correlation). Thus, tampon specimens are an easy method for assessment of genital HPV infection.
UR - http://www.scopus.com/inward/record.url?scp=0026521360&partnerID=8YFLogxK
U2 - 10.1093/infdis/165.6.1103
DO - 10.1093/infdis/165.6.1103
M3 - Article
C2 - 1316411
AN - SCOPUS:0026521360
SN - 0022-1899
VL - 165
SP - 1103
EP - 1106
JO - The Journal of Infectious Diseases
JF - The Journal of Infectious Diseases
IS - 6
ER -
