TamA interacts with LeuB, the homologue of Saccharomyces cerevisiae Leu3p, to regulate gdhA expression in Aspergillus nidulans

R. Polotnianka, B. J. Monahan, M. J. Hynes, M. A. Davis

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11 Citations (Scopus)

Abstract

Previous studies have shown that expression of the gdhA gene, encoding NADP-linked glutamate dehydrogenase (NADP-GDH), in Aspergillus nidulans is regulated by the major nitrogen regulatory protein AreA and its co-activator TamA. We show here that loss of TamA function has a more severe effect on the levels of gdhA expression than loss of AreA function. Using TamA as the bait in a yeast two-hybrid screen, we have identified a second protein that interacts with TamA. Sequencing analysis and functional studies have shown that this protein, designated LeuB, is a transcriptional activator with similar function to the homologous Leu3p of Saccharomyces cerevisiae. Inactivation of leuB revealed that this gene is involved in the regulation of gdhA, and an are A; leuB double mutant was shown to have similar NADP-GDH levels to a tamA single mutant. The requirement for TamA function to promote gdhA expression is likely to be due to its dual interaction with AreA and LeuB.

Original languageEnglish
Pages (from-to)452-459
Number of pages8
JournalMolecular Genetics and Genomics
Volume272
Issue number4
DOIs
Publication statusPublished - Nov 2004
Externally publishedYes

Keywords

  • Ammonium assimilation
  • Gene regulation
  • Leucine biosynthesis
  • NADP-linked glutamate dehydrogenase

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