Tailored emulsion-templated porous polymer scaffolds for iPSC-derived human neural precursor cell culture

Ashley R. Murphy, Irene Ghobrial, Pegah Jamshidi, Andrew Laslett, Carmel M. O'Brien, Neil R. Cameron

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

The work here describes the synthesis of tailor-made, porous, polymeric materials with elastic moduli in the range associated with mammalian brain tissue (0.1-24 kPa). Three new emulsion-templated porous polymer materials (polyHIPEs) were synthesised by thiol-ene photopolymerisation from hexanediol diacrylate (HDDA) and polyethylene glycol diacrylate (PEGDA) crosslinkers and compared with a previously reported material prepared from trimethylolpropane triacrylate (TMPTA). The materials were found to have an average pore diameter of 30-63 μm and a porosity of 77% and above. PEGDA crosslinked materials at 80 and 85% porosity, when swollen in PBS at 37 °C, were found to have an elastic modulus of 18 and 9.0 kPa respectively. PEGDA crosslinked materials were also found to have a swelling ratio of 700% in PBS at 37 °C. PEGDA crosslinked materials had improved visible light transmission properties when compared to TMPTA crosslinked materials under a bright field microscope. All materials were shown via hematoxylin and eosin staining to support the infiltration and attachment of induced pluripotent stem cell (iPSC)-derived human neural progenitor cells (hNPCs). HNPCs on all materials were demonstrated in short term 3D cultures to maintain a phenotype consistent with early neural lineage specification via immunohistochemical staining for the intermediate filament protein vimentin.

Original languageEnglish
Pages (from-to)6617-6627
Number of pages11
JournalPolymer Chemistry
Volume8
Issue number43
DOIs
Publication statusPublished - 20 Nov 2017

Cite this

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title = "Tailored emulsion-templated porous polymer scaffolds for iPSC-derived human neural precursor cell culture",
abstract = "The work here describes the synthesis of tailor-made, porous, polymeric materials with elastic moduli in the range associated with mammalian brain tissue (0.1-24 kPa). Three new emulsion-templated porous polymer materials (polyHIPEs) were synthesised by thiol-ene photopolymerisation from hexanediol diacrylate (HDDA) and polyethylene glycol diacrylate (PEGDA) crosslinkers and compared with a previously reported material prepared from trimethylolpropane triacrylate (TMPTA). The materials were found to have an average pore diameter of 30-63 μm and a porosity of 77{\%} and above. PEGDA crosslinked materials at 80 and 85{\%} porosity, when swollen in PBS at 37 °C, were found to have an elastic modulus of 18 and 9.0 kPa respectively. PEGDA crosslinked materials were also found to have a swelling ratio of 700{\%} in PBS at 37 °C. PEGDA crosslinked materials had improved visible light transmission properties when compared to TMPTA crosslinked materials under a bright field microscope. All materials were shown via hematoxylin and eosin staining to support the infiltration and attachment of induced pluripotent stem cell (iPSC)-derived human neural progenitor cells (hNPCs). HNPCs on all materials were demonstrated in short term 3D cultures to maintain a phenotype consistent with early neural lineage specification via immunohistochemical staining for the intermediate filament protein vimentin.",
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Tailored emulsion-templated porous polymer scaffolds for iPSC-derived human neural precursor cell culture. / Murphy, Ashley R.; Ghobrial, Irene; Jamshidi, Pegah; Laslett, Andrew; O'Brien, Carmel M.; Cameron, Neil R.

In: Polymer Chemistry, Vol. 8, No. 43, 20.11.2017, p. 6617-6627.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Ghobrial, Irene

AU - Jamshidi, Pegah

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AU - O'Brien, Carmel M.

AU - Cameron, Neil R.

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