TY - JOUR
T1 - TA-cloning vectors for rapid and cheap cloning of zebrafish transgenesis constructs
AU - Miles, Lee B.
AU - Verkade, Heather
PY - 2014/6/1
Y1 - 2014/6/1
N2 - Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and final Tol2-transgenesis vectors for zebra fish transgenesis, as well as a TA-cloning expression vector, pCS2 + TA, for rapid generation of new expression plasmids.
AB - Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and final Tol2-transgenesis vectors for zebra fish transgenesis, as well as a TA-cloning expression vector, pCS2 + TA, for rapid generation of new expression plasmids.
UR - http://www.scopus.com/inward/record.url?scp=84902331621&partnerID=8YFLogxK
U2 - 10.1089/zeb.2013.0954
DO - 10.1089/zeb.2013.0954
M3 - Comment / Debate
C2 - 24918328
AN - SCOPUS:84902331621
SN - 1545-8547
VL - 11
SP - 281
EP - 282
JO - Zebrafish
JF - Zebrafish
IS - 3
ER -