TA-cloning vectors for rapid and cheap cloning of zebrafish transgenesis constructs

Lee B. Miles, Heather Verkade

Research output: Contribution to journalComment / DebateOtherpeer-review

4 Citations (Scopus)

Abstract

Here we report the generation and use of three multisite Gateway TA-cloning plasmids, p5E-TA, pME-TA, and p3E-TA, to enable rapid and cheap construction of new entry vectors and final Tol2-transgenesis vectors for zebra fish transgenesis, as well as a TA-cloning expression vector, pCS2 + TA, for rapid generation of new expression plasmids.
Original languageEnglish
Pages (from-to)281-282
Number of pages2
JournalZebrafish
Volume11
Issue number3
DOIs
Publication statusPublished - 1 Jun 2014

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