T lymphocytes from granulocyte colony-stimulating factor(-/-) mice produce large quantities of interferon-γ in a chronic infection model

S. I. Mannering, Y. Zhan, B. Gilbertson, G. J. Lieschke, C. Cheers

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9 Citations (Scopus)

Abstract

Little is known about the role of granulocyte colony-stimulating factor (G-CSF) in the response to chronic bacterial infections. To address this we infected G-CSF knock out (G-CSF(-/-)) mice with Mycobacterium avium. Infection was not exacerbated in G-CSF(-/-) mice despite a deficiency in the total bone marrow cells, colony-forming haemopoietic cells, granulocytes and monocyte precursors in the bone marrow. Peritoneal cells from G-CSF(-/-) produced less nitric oxide (NO) upon culture in vitro with antigen than did wild-type (WT) cells. Unexpectedly, T cells from infected G-CSF(-/-) mice were able to produce significantly more interferon-γ (IFN-γ) than the wild type (WT) controls. T cells from G-CSF(-/-) mice still produced more IFN-γ even when in vitro NO production was inhibited, while enzyme-linked immunospot assay (ELISPOT) assays showed more IFN-γ-producing cells in the G-CSF(-/-) mice. This was confirmed by intracellular cytokine staining (ICCS), which showed that there were more IFN-γ producing T cells in vivo in the G-CSF(-/-) than the WT controls following M. avium infection. It is possible that a deficit of NO in vivo allows T cells to develop a higher IFN-γ-producing phenotype. Thus we show a novel relationship between G-CSF and IFN-γ production by T cells revealed in this chronic bacterial infection model.

Original languageEnglish
Pages (from-to)132-139
Number of pages8
JournalImmunology
Volume101
Issue number1
DOIs
Publication statusPublished - 2000
Externally publishedYes

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