T-cell receptor-optimized peptide skewing of the T-cell repertoire can enhance antigen targeting

Julia Ekeruche-Makinde, Mathew Clement, David K. Cole, Emily S J Edwards, Kristin Ladell, John J. Miles, Katherine K. Matthews, Anna Fuller, Katy A. Lloyd, Florian Madura, Garry M. Dolton, Johanne Pentier, Anna Lissina, Emma Gostick, Tiffany K. Baxter, Brian M. Baker, Pierre J. Rizkallah, David A. Price, Linda Wooldridge, Andrew K. Sewell

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

Background: Current peptide vaccines may select suboptimal antigen-specific T-cells from polyclonal populations. Results:Acombinatorial peptide library screen was used to generate an optimal ligand that could preferentially activate a known effective T-cell clonotype. Conclusion: Rationally designed altered peptide ligands may enable the preferential selection of high quality, antigen-sensitive T-cell clonotypes. Significance: This proof-of-principle study could facilitate the development of more effective peptide vaccination strategies.

Original languageEnglish
Pages (from-to)37269-37281
Number of pages13
JournalThe Journal of Biological Chemistry
Volume287
Issue number44
DOIs
Publication statusPublished - 26 Oct 2012
Externally publishedYes

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