T cell receptor α-chain usage can determine ant igen-selected tcr 3-chain repertoire diversity

S. J. Turner, F. R. Carbone

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Certain antigen-specific responses can give rise to simple V-region bias in the T cell receptor (TCR) repertoire while others can be dominated by a single TCR donotype repeatedly isolated from different individuals. Cytotoxic T lymphocytes (CTL) specific for a K b -binding determinant from the Herpes Simplex Virus (HSV) glycoprotein B (gB), preferentially express a dominant Vβ10 TCR γ-chain subset with extensive junctional conservation. We examined the contribution of the TCR α-chain to the diversity of the gB-specific CTL response. The TCR γ-cliains were found to exhibit minimal bias in either their sequence selection or their preferential /3-chain pairing. When the gB-specific CTL were forced to use a single TCR a-chain, the T cells exhibited a severely limited form of β-chain selection. The response was now dominated by a single Vβ10 β-chain sequence (Vβ10/Jβ2.6) which showed a conserved junctional motif found in the more diverse non-transgenic T cell population.

Original languageEnglish
JournalFASEB Journal
Volume10
Issue number6
Publication statusPublished - 1 Dec 1996
Externally publishedYes

Cite this

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title = "T cell receptor α-chain usage can determine ant igen-selected tcr 3-chain repertoire diversity",
abstract = "Certain antigen-specific responses can give rise to simple V-region bias in the T cell receptor (TCR) repertoire while others can be dominated by a single TCR donotype repeatedly isolated from different individuals. Cytotoxic T lymphocytes (CTL) specific for a K b -binding determinant from the Herpes Simplex Virus (HSV) glycoprotein B (gB), preferentially express a dominant Vβ10 TCR γ-chain subset with extensive junctional conservation. We examined the contribution of the TCR α-chain to the diversity of the gB-specific CTL response. The TCR γ-cliains were found to exhibit minimal bias in either their sequence selection or their preferential /3-chain pairing. When the gB-specific CTL were forced to use a single TCR a-chain, the T cells exhibited a severely limited form of β-chain selection. The response was now dominated by a single Vβ10 β-chain sequence (Vβ10/Jβ2.6) which showed a conserved junctional motif found in the more diverse non-transgenic T cell population.",
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T cell receptor α-chain usage can determine ant igen-selected tcr 3-chain repertoire diversity. / Turner, S. J.; Carbone, F. R.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - T cell receptor α-chain usage can determine ant igen-selected tcr 3-chain repertoire diversity

AU - Turner, S. J.

AU - Carbone, F. R.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - Certain antigen-specific responses can give rise to simple V-region bias in the T cell receptor (TCR) repertoire while others can be dominated by a single TCR donotype repeatedly isolated from different individuals. Cytotoxic T lymphocytes (CTL) specific for a K b -binding determinant from the Herpes Simplex Virus (HSV) glycoprotein B (gB), preferentially express a dominant Vβ10 TCR γ-chain subset with extensive junctional conservation. We examined the contribution of the TCR α-chain to the diversity of the gB-specific CTL response. The TCR γ-cliains were found to exhibit minimal bias in either their sequence selection or their preferential /3-chain pairing. When the gB-specific CTL were forced to use a single TCR a-chain, the T cells exhibited a severely limited form of β-chain selection. The response was now dominated by a single Vβ10 β-chain sequence (Vβ10/Jβ2.6) which showed a conserved junctional motif found in the more diverse non-transgenic T cell population.

AB - Certain antigen-specific responses can give rise to simple V-region bias in the T cell receptor (TCR) repertoire while others can be dominated by a single TCR donotype repeatedly isolated from different individuals. Cytotoxic T lymphocytes (CTL) specific for a K b -binding determinant from the Herpes Simplex Virus (HSV) glycoprotein B (gB), preferentially express a dominant Vβ10 TCR γ-chain subset with extensive junctional conservation. We examined the contribution of the TCR α-chain to the diversity of the gB-specific CTL response. The TCR γ-cliains were found to exhibit minimal bias in either their sequence selection or their preferential /3-chain pairing. When the gB-specific CTL were forced to use a single TCR a-chain, the T cells exhibited a severely limited form of β-chain selection. The response was now dominated by a single Vβ10 β-chain sequence (Vβ10/Jβ2.6) which showed a conserved junctional motif found in the more diverse non-transgenic T cell population.

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