T Cell Help Amplifies Innate Signals in CD8+ DCs for Optimal CD8+ T Cell Priming

Marie Greyer, Paul G Whitney, Angus T. Stock, Gayle M. Davey, Christina Tebartz, Annabell Bachem, Justine D. Mintern, Richard A Strugnell, Stephen J. Turner, Thomas Gebhardt, Meredith O'Keeffe, William R Heath, Sammy Bedoui

Research output: Contribution to journalArticleResearchpeer-review

Abstract

DCs often require stimulation from CD4+ T cells to propagate CD8+ T cell responses, but precisely how T cell help optimizes the priming capacity of DCs and why this appears to differ between varying types of CD8+ T cell immunity remains unclear. We show that CD8+ T cell priming upon HSV-1 skin infection depended on DCs receiving stimulation from both IFN-α/β and CD4+ T cells to provide IL-15. This was not an additive effect but resulted from CD4+ T cells amplifying DC production of IL-15 in response to IFN-α/β. We also observed that increased innate stimulation reversed the helper dependence of CD8+ T cell priming and that the innate stimulus, rather than the CD4+ T cells themselves, determined how "help'" was integrated into the priming response by DCs. These findings identify T cell help as a flexible means to amplify varying suboptimal innate signals in DCs.

Original languageEnglish
Pages (from-to)586-597
Number of pages12
JournalCell Reports
Volume14
Issue number3
DOIs
Publication statusPublished - 26 Jan 2016

Cite this

Greyer, M., Whitney, P. G., Stock, A. T., Davey, G. M., Tebartz, C., Bachem, A., ... Bedoui, S. (2016). T Cell Help Amplifies Innate Signals in CD8+ DCs for Optimal CD8+ T Cell Priming. Cell Reports, 14(3), 586-597. https://doi.org/10.1016/j.celrep.2015.12.058
Greyer, Marie ; Whitney, Paul G ; Stock, Angus T. ; Davey, Gayle M. ; Tebartz, Christina ; Bachem, Annabell ; Mintern, Justine D. ; Strugnell, Richard A ; Turner, Stephen J. ; Gebhardt, Thomas ; O'Keeffe, Meredith ; Heath, William R ; Bedoui, Sammy. / T Cell Help Amplifies Innate Signals in CD8+ DCs for Optimal CD8+ T Cell Priming. In: Cell Reports. 2016 ; Vol. 14, No. 3. pp. 586-597.
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Greyer, M, Whitney, PG, Stock, AT, Davey, GM, Tebartz, C, Bachem, A, Mintern, JD, Strugnell, RA, Turner, SJ, Gebhardt, T, O'Keeffe, M, Heath, WR & Bedoui, S 2016, 'T Cell Help Amplifies Innate Signals in CD8+ DCs for Optimal CD8+ T Cell Priming' Cell Reports, vol. 14, no. 3, pp. 586-597. https://doi.org/10.1016/j.celrep.2015.12.058

T Cell Help Amplifies Innate Signals in CD8+ DCs for Optimal CD8+ T Cell Priming. / Greyer, Marie; Whitney, Paul G; Stock, Angus T.; Davey, Gayle M.; Tebartz, Christina; Bachem, Annabell; Mintern, Justine D.; Strugnell, Richard A; Turner, Stephen J.; Gebhardt, Thomas; O'Keeffe, Meredith; Heath, William R; Bedoui, Sammy.

In: Cell Reports, Vol. 14, No. 3, 26.01.2016, p. 586-597.

Research output: Contribution to journalArticleResearchpeer-review

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Greyer M, Whitney PG, Stock AT, Davey GM, Tebartz C, Bachem A et al. T Cell Help Amplifies Innate Signals in CD8+ DCs for Optimal CD8+ T Cell Priming. Cell Reports. 2016 Jan 26;14(3):586-597. https://doi.org/10.1016/j.celrep.2015.12.058