T cell autoreactivity directed toward CD1c itself rather than toward carried self lipids

Kwok S. Wun, Josephine F. Reijneveld, Tan Yun Cheng, Kristin Ladell, Adam P. Uldrich, Jérôme Le Nours, Kelly L. Miners, James E. McLaren, Emma J. Grant, Oscar L. Haigh, Thomas S. Watkins, Sara Suliman, Sarah Iwany, Judith Jimenez, Roger Calderon, Kattya L. Tamara, Segundo R. Leon, Megan B. Murray, Jacob A. Mayfield, John D. Altman & 8 others Anthony W. Purcell, John J. Miles, Dale I. Godfrey, Stephanie Gras, David A. Price, Ildiko Van Rhijn, D. Branch Moody, Jamie Rossjohn

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The hallmark function of αβ T cell antigen receptors (TCRs) involves the highly specific co-recognition of a major histocompatibility complex molecule and its carried peptide. However, the molecular basis of the interactions of TCRs with the lipid antigen-presenting molecule CD1c is unknown. We identified frequent staining of human T cells with CD1c tetramers across numerous subjects. Whereas TCRs typically show high specificity for antigen, both tetramer binding and autoreactivity occurred with CD1c in complex with numerous, chemically diverse self lipids. Such extreme polyspecificity was attributable to binding of the TCR over the closed surface of CD1c, with the TCR covering the portal where lipids normally protrude. The TCR essentially failed to contact lipids because they were fully seated within CD1c. These data demonstrate the sequestration of lipids within CD1c as a mechanism of autoreactivity and point to small lipid size as a determinant of autoreactive T cell responses.

Original languageEnglish
Pages (from-to)397-406
Number of pages10
JournalNature Immunology
Volume19
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018

Cite this

Wun, Kwok S. ; Reijneveld, Josephine F. ; Cheng, Tan Yun ; Ladell, Kristin ; Uldrich, Adam P. ; Le Nours, Jérôme ; Miners, Kelly L. ; McLaren, James E. ; Grant, Emma J. ; Haigh, Oscar L. ; Watkins, Thomas S. ; Suliman, Sara ; Iwany, Sarah ; Jimenez, Judith ; Calderon, Roger ; Tamara, Kattya L. ; Leon, Segundo R. ; Murray, Megan B. ; Mayfield, Jacob A. ; Altman, John D. ; Purcell, Anthony W. ; Miles, John J. ; Godfrey, Dale I. ; Gras, Stephanie ; Price, David A. ; Van Rhijn, Ildiko ; Moody, D. Branch ; Rossjohn, Jamie. / T cell autoreactivity directed toward CD1c itself rather than toward carried self lipids. In: Nature Immunology. 2018 ; Vol. 19, No. 4. pp. 397-406.
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title = "T cell autoreactivity directed toward CD1c itself rather than toward carried self lipids",
abstract = "The hallmark function of αβ T cell antigen receptors (TCRs) involves the highly specific co-recognition of a major histocompatibility complex molecule and its carried peptide. However, the molecular basis of the interactions of TCRs with the lipid antigen-presenting molecule CD1c is unknown. We identified frequent staining of human T cells with CD1c tetramers across numerous subjects. Whereas TCRs typically show high specificity for antigen, both tetramer binding and autoreactivity occurred with CD1c in complex with numerous, chemically diverse self lipids. Such extreme polyspecificity was attributable to binding of the TCR over the closed surface of CD1c, with the TCR covering the portal where lipids normally protrude. The TCR essentially failed to contact lipids because they were fully seated within CD1c. These data demonstrate the sequestration of lipids within CD1c as a mechanism of autoreactivity and point to small lipid size as a determinant of autoreactive T cell responses.",
author = "Wun, {Kwok S.} and Reijneveld, {Josephine F.} and Cheng, {Tan Yun} and Kristin Ladell and Uldrich, {Adam P.} and {Le Nours}, J{\'e}r{\^o}me and Miners, {Kelly L.} and McLaren, {James E.} and Grant, {Emma J.} and Haigh, {Oscar L.} and Watkins, {Thomas S.} and Sara Suliman and Sarah Iwany and Judith Jimenez and Roger Calderon and Tamara, {Kattya L.} and Leon, {Segundo R.} and Murray, {Megan B.} and Mayfield, {Jacob A.} and Altman, {John D.} and Purcell, {Anthony W.} and Miles, {John J.} and Godfrey, {Dale I.} and Stephanie Gras and Price, {David A.} and {Van Rhijn}, Ildiko and Moody, {D. Branch} and Jamie Rossjohn",
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Wun, KS, Reijneveld, JF, Cheng, TY, Ladell, K, Uldrich, AP, Le Nours, J, Miners, KL, McLaren, JE, Grant, EJ, Haigh, OL, Watkins, TS, Suliman, S, Iwany, S, Jimenez, J, Calderon, R, Tamara, KL, Leon, SR, Murray, MB, Mayfield, JA, Altman, JD, Purcell, AW, Miles, JJ, Godfrey, DI, Gras, S, Price, DA, Van Rhijn, I, Moody, DB & Rossjohn, J 2018, 'T cell autoreactivity directed toward CD1c itself rather than toward carried self lipids' Nature Immunology, vol. 19, no. 4, pp. 397-406. https://doi.org/10.1038/s41590-018-0065-7

T cell autoreactivity directed toward CD1c itself rather than toward carried self lipids. / Wun, Kwok S.; Reijneveld, Josephine F.; Cheng, Tan Yun; Ladell, Kristin; Uldrich, Adam P.; Le Nours, Jérôme; Miners, Kelly L.; McLaren, James E.; Grant, Emma J.; Haigh, Oscar L.; Watkins, Thomas S.; Suliman, Sara; Iwany, Sarah; Jimenez, Judith; Calderon, Roger; Tamara, Kattya L.; Leon, Segundo R.; Murray, Megan B.; Mayfield, Jacob A.; Altman, John D.; Purcell, Anthony W.; Miles, John J.; Godfrey, Dale I.; Gras, Stephanie; Price, David A.; Van Rhijn, Ildiko; Moody, D. Branch; Rossjohn, Jamie.

In: Nature Immunology, Vol. 19, No. 4, 01.04.2018, p. 397-406.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Godfrey, Dale I.

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