T and B cell markers in dried blood spots of neonates with congenital cytomegalovirus infection

B cell numbers at birth are associated with long-term outcomes

Roberta Rovito, Marjolein J. Korndewal, Menno C. Van Zelm, Dimitrios Ziagkos, Els Wessels, Mirjam Van Der Burg, Aloys C M Kroes, Anton W. Langerak, Ann C T M Vossen

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Congenital CMV infection (cCMV) is the most common congenital infection that can cause long-term impairment (LTI). The pathogenesis of LTI is not completely understood. Fetal immunity may play a role in controlling the infection and preventing LTI, although immune activation may also contribute to fetal immunopathology. In this study, we analyzed various molecular markers of Tand B cell numbers in neonatal dried blood spots of 99 children with cCMV and 54 children without cCMV: δRec-ψJα signal joints on TCR excision circles, intron recombination signal sequence k-deleting element signal joints on Igκ-deleting recombination excision circles, genomic intron recombination signal sequence k-deleting element coding joint, genomic Vδ1-Jδ1, and Vδ2-Jδ1 rearrangements. Of this cohort, clinical symptoms at birth and LTI at 6 y of age were recorded. Neonates with cCMV had fewer TCR excision circles in their blood than non-infected controls. Furthermore, cCMV infection was associated with increased numbers of γδ T cells and B cells, and these numbers were positively correlated with CMV viral load in the dried blood spots. Infected children with a better long-term outcome had higher numbers of B cells atbirththanthosewhodeveloped LTI; no difference in B cell replication was observed. The potential protective role of B cells in controlling cCMV-related disease and the clinical value of this marker as a predictor of long-term outcome merit further evaluation.

Original languageEnglish
Pages (from-to)102-109
Number of pages8
JournalJournal of Immunology
Volume198
Issue number1
DOIs
Publication statusPublished - 1 Jan 2017

Cite this

Rovito, Roberta ; Korndewal, Marjolein J. ; Van Zelm, Menno C. ; Ziagkos, Dimitrios ; Wessels, Els ; Van Der Burg, Mirjam ; Kroes, Aloys C M ; Langerak, Anton W. ; Vossen, Ann C T M. / T and B cell markers in dried blood spots of neonates with congenital cytomegalovirus infection : B cell numbers at birth are associated with long-term outcomes. In: Journal of Immunology. 2017 ; Vol. 198, No. 1. pp. 102-109.
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title = "T and B cell markers in dried blood spots of neonates with congenital cytomegalovirus infection: B cell numbers at birth are associated with long-term outcomes",
abstract = "Congenital CMV infection (cCMV) is the most common congenital infection that can cause long-term impairment (LTI). The pathogenesis of LTI is not completely understood. Fetal immunity may play a role in controlling the infection and preventing LTI, although immune activation may also contribute to fetal immunopathology. In this study, we analyzed various molecular markers of Tand B cell numbers in neonatal dried blood spots of 99 children with cCMV and 54 children without cCMV: δRec-ψJα signal joints on TCR excision circles, intron recombination signal sequence k-deleting element signal joints on Igκ-deleting recombination excision circles, genomic intron recombination signal sequence k-deleting element coding joint, genomic Vδ1-Jδ1, and Vδ2-Jδ1 rearrangements. Of this cohort, clinical symptoms at birth and LTI at 6 y of age were recorded. Neonates with cCMV had fewer TCR excision circles in their blood than non-infected controls. Furthermore, cCMV infection was associated with increased numbers of γδ T cells and B cells, and these numbers were positively correlated with CMV viral load in the dried blood spots. Infected children with a better long-term outcome had higher numbers of B cells atbirththanthosewhodeveloped LTI; no difference in B cell replication was observed. The potential protective role of B cells in controlling cCMV-related disease and the clinical value of this marker as a predictor of long-term outcome merit further evaluation.",
author = "Roberta Rovito and Korndewal, {Marjolein J.} and {Van Zelm}, {Menno C.} and Dimitrios Ziagkos and Els Wessels and {Van Der Burg}, Mirjam and Kroes, {Aloys C M} and Langerak, {Anton W.} and Vossen, {Ann C T M}",
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T and B cell markers in dried blood spots of neonates with congenital cytomegalovirus infection : B cell numbers at birth are associated with long-term outcomes. / Rovito, Roberta; Korndewal, Marjolein J.; Van Zelm, Menno C.; Ziagkos, Dimitrios; Wessels, Els; Van Der Burg, Mirjam; Kroes, Aloys C M; Langerak, Anton W.; Vossen, Ann C T M.

In: Journal of Immunology, Vol. 198, No. 1, 01.01.2017, p. 102-109.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Rovito, Roberta

AU - Korndewal, Marjolein J.

AU - Van Zelm, Menno C.

AU - Ziagkos, Dimitrios

AU - Wessels, Els

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AU - Kroes, Aloys C M

AU - Langerak, Anton W.

AU - Vossen, Ann C T M

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