Abstract
Congenital CMV infection (cCMV) is the most common congenital infection that can cause long-term impairment (LTI). The pathogenesis of LTI is not completely understood. Fetal immunity may play a role in controlling the infection and preventing LTI, although immune activation may also contribute to fetal immunopathology. In this study, we analyzed various molecular markers of Tand B cell numbers in neonatal dried blood spots of 99 children with cCMV and 54 children without cCMV: δRec-ψJα signal joints on TCR excision circles, intron recombination signal sequence k-deleting element signal joints on Igκ-deleting recombination excision circles, genomic intron recombination signal sequence k-deleting element coding joint, genomic Vδ1-Jδ1, and Vδ2-Jδ1 rearrangements. Of this cohort, clinical symptoms at birth and LTI at 6 y of age were recorded. Neonates with cCMV had fewer TCR excision circles in their blood than non-infected controls. Furthermore, cCMV infection was associated with increased numbers of γδ T cells and B cells, and these numbers were positively correlated with CMV viral load in the dried blood spots. Infected children with a better long-term outcome had higher numbers of B cells atbirththanthosewhodeveloped LTI; no difference in B cell replication was observed. The potential protective role of B cells in controlling cCMV-related disease and the clinical value of this marker as a predictor of long-term outcome merit further evaluation.
| Original language | English |
|---|---|
| Pages (from-to) | 102-109 |
| Number of pages | 8 |
| Journal | Journal of Immunology |
| Volume | 198 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1 Jan 2017 |
Cite this
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T and B cell markers in dried blood spots of neonates with congenital cytomegalovirus infection : B cell numbers at birth are associated with long-term outcomes. / Rovito, Roberta; Korndewal, Marjolein J.; Van Zelm, Menno C.; Ziagkos, Dimitrios; Wessels, Els; Van Der Burg, Mirjam; Kroes, Aloys C M; Langerak, Anton W.; Vossen, Ann C T M.
In: Journal of Immunology, Vol. 198, No. 1, 01.01.2017, p. 102-109.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - T and B cell markers in dried blood spots of neonates with congenital cytomegalovirus infection
T2 - B cell numbers at birth are associated with long-term outcomes
AU - Rovito, Roberta
AU - Korndewal, Marjolein J.
AU - Van Zelm, Menno C.
AU - Ziagkos, Dimitrios
AU - Wessels, Els
AU - Van Der Burg, Mirjam
AU - Kroes, Aloys C M
AU - Langerak, Anton W.
AU - Vossen, Ann C T M
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Congenital CMV infection (cCMV) is the most common congenital infection that can cause long-term impairment (LTI). The pathogenesis of LTI is not completely understood. Fetal immunity may play a role in controlling the infection and preventing LTI, although immune activation may also contribute to fetal immunopathology. In this study, we analyzed various molecular markers of Tand B cell numbers in neonatal dried blood spots of 99 children with cCMV and 54 children without cCMV: δRec-ψJα signal joints on TCR excision circles, intron recombination signal sequence k-deleting element signal joints on Igκ-deleting recombination excision circles, genomic intron recombination signal sequence k-deleting element coding joint, genomic Vδ1-Jδ1, and Vδ2-Jδ1 rearrangements. Of this cohort, clinical symptoms at birth and LTI at 6 y of age were recorded. Neonates with cCMV had fewer TCR excision circles in their blood than non-infected controls. Furthermore, cCMV infection was associated with increased numbers of γδ T cells and B cells, and these numbers were positively correlated with CMV viral load in the dried blood spots. Infected children with a better long-term outcome had higher numbers of B cells atbirththanthosewhodeveloped LTI; no difference in B cell replication was observed. The potential protective role of B cells in controlling cCMV-related disease and the clinical value of this marker as a predictor of long-term outcome merit further evaluation.
AB - Congenital CMV infection (cCMV) is the most common congenital infection that can cause long-term impairment (LTI). The pathogenesis of LTI is not completely understood. Fetal immunity may play a role in controlling the infection and preventing LTI, although immune activation may also contribute to fetal immunopathology. In this study, we analyzed various molecular markers of Tand B cell numbers in neonatal dried blood spots of 99 children with cCMV and 54 children without cCMV: δRec-ψJα signal joints on TCR excision circles, intron recombination signal sequence k-deleting element signal joints on Igκ-deleting recombination excision circles, genomic intron recombination signal sequence k-deleting element coding joint, genomic Vδ1-Jδ1, and Vδ2-Jδ1 rearrangements. Of this cohort, clinical symptoms at birth and LTI at 6 y of age were recorded. Neonates with cCMV had fewer TCR excision circles in their blood than non-infected controls. Furthermore, cCMV infection was associated with increased numbers of γδ T cells and B cells, and these numbers were positively correlated with CMV viral load in the dried blood spots. Infected children with a better long-term outcome had higher numbers of B cells atbirththanthosewhodeveloped LTI; no difference in B cell replication was observed. The potential protective role of B cells in controlling cCMV-related disease and the clinical value of this marker as a predictor of long-term outcome merit further evaluation.
UR - http://www.scopus.com/inward/record.url?scp=85007008187&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1601182
DO - 10.4049/jimmunol.1601182
M3 - Article
VL - 198
SP - 102
EP - 109
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 1
ER -