TY - JOUR
T1 - Systemic absorption of antioxidants from mulberry (Morus alba L) leaf extracts using an in situ rat intestinal preparation
AU - Lee, Chooi Yeng
AU - Sim, Si Mui
AU - Cheng, Hwee Ming
N1 - Funding Information:
This work was supported in part by the Ministry of Sciences, Technology, and Innovation through an IRPA grant (06-02-03-0165). We thank Jelan Zainal from the Department of Animal Science, Faculty of Agriculture, Universiti Putra Malaysia, for kindly providing the mulberry leaves.
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2007/8
Y1 - 2007/8
N2 - Consumption of antioxidant-rich foods is thought to be capable of increasing our body antioxidant levels, but little is known about their systemic bioavailability. The aim of this study was to demonstrate the systemic absorption of antioxidants from the aqueous and ethanol extracts of mulberry leaves by using an in situ rat intestinal preparation. After an overnight fast, the carotid artery of an anesthetized rat was cannulated, followed by isolation of a short length (8-13 cm) of the duodenum, jejunum, or ileum. Blood samples were collected from the artery before and at various times after administration of the extract or vehicle into the isolated intestinal segment. The collected plasma samples were assayed for their total antioxidant activity (TAA). There was a transient increase in the plasma TAA for animals given the aqueous extract at the ileum, but not when given at the duodenum or jejunum. This suggests that the water-extracted antioxidants need to be hydrolyzed, probably by bacteria, before absorption. For animals given the ethanol extract, significant increase in the plasma TAA occurred at different times for duodenal, jejunal, and ileal segments. This indicates that the ethanol-extracted antioxidants are more readily absorbed. The absorption of these antioxidants probably involves several different mechanisms, and may be influenced by the vehicle of administration. In conclusion, the antioxidants in mulberry leaves are absorbed to different extents throughout the small intestine of rats. Furthermore, this in situ preparation may be used to screen for systemic bioavailability of antioxidants in herbal samples.
AB - Consumption of antioxidant-rich foods is thought to be capable of increasing our body antioxidant levels, but little is known about their systemic bioavailability. The aim of this study was to demonstrate the systemic absorption of antioxidants from the aqueous and ethanol extracts of mulberry leaves by using an in situ rat intestinal preparation. After an overnight fast, the carotid artery of an anesthetized rat was cannulated, followed by isolation of a short length (8-13 cm) of the duodenum, jejunum, or ileum. Blood samples were collected from the artery before and at various times after administration of the extract or vehicle into the isolated intestinal segment. The collected plasma samples were assayed for their total antioxidant activity (TAA). There was a transient increase in the plasma TAA for animals given the aqueous extract at the ileum, but not when given at the duodenum or jejunum. This suggests that the water-extracted antioxidants need to be hydrolyzed, probably by bacteria, before absorption. For animals given the ethanol extract, significant increase in the plasma TAA occurred at different times for duodenal, jejunal, and ileal segments. This indicates that the ethanol-extracted antioxidants are more readily absorbed. The absorption of these antioxidants probably involves several different mechanisms, and may be influenced by the vehicle of administration. In conclusion, the antioxidants in mulberry leaves are absorbed to different extents throughout the small intestine of rats. Furthermore, this in situ preparation may be used to screen for systemic bioavailability of antioxidants in herbal samples.
KW - Antioxidants
KW - Bioavailability
KW - Intestinal absorption
KW - Mulberry (Morus alba L)
KW - Rat
KW - Total antioxidant activity
UR - http://www.scopus.com/inward/record.url?scp=34547150540&partnerID=8YFLogxK
U2 - 10.1016/j.nutres.2007.06.004
DO - 10.1016/j.nutres.2007.06.004
M3 - Article
AN - SCOPUS:34547150540
SN - 0271-5317
VL - 27
SP - 492
EP - 497
JO - Nutrition Research
JF - Nutrition Research
IS - 8
ER -