Synthetic mRNA reprogramming of human fibroblast cells

Jun Liu, Paul J. Verma

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

6 Citations (Scopus)

Abstract

Reprogramming of somatic cells, such as skin fibroblasts, to pluripotency was first achieved by forced expression of four transcription factors using integrating retroviral or lentiviral vectors, which result in integration of exogenous DNA into cellular genome and present a formidable barrier to therapeutic application of induced pluripotent stem cells (iPSCs). To facilitate the translation of iPSC technology to clinical practice, mRNA reprogramming method that generates transgene-free iPSCs is a safe and efficient method, eliminating bio-containment concerns associated with viral vectors, as well as the need for weeks of screening of cells to confirm that viral material has been completely eliminated during cell passaging.
Original languageEnglish
Title of host publicationCell reprogramming
Subtitle of host publicationMethod and protocols
EditorsPaul J. Verma, Huseyin Sumer
Place of PublicationNew York
PublisherHumana Press
Chapter2
Pages17-28
Number of pages12
Volume1330
ISBN (Electronic)9781493928484
ISBN (Print)9781493928477
DOIs
Publication statusPublished - 2015

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Reprogramming
  • Transgene-free
  • Induced pluripotent stem cells
  • Modifi ed mRNA
  • Transfection

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