Synthesis of siRNA polyplexes adopting a combination of raft polymerization and thiol-ene chemistry

David Valade, Cyrille Boyer, Thomas Paul Davis, Volga Bulmus

Research output: Contribution to journalArticleResearchpeer-review

36 Citations (Scopus)

Abstract

Block copolymers of allyl methacrylate and N-(2-hydroxypropyl) methacrylamide (HPMA) with different block lengths have been synthesized by reversible additionfragmentation chain transfer polymerization. Allyl groups were modified with cysteamine, via a thiol-ene photoreaction, with a high efficiency ( 100 ) as evidenced by NMR spectroscopy, yielding cationic copolymers of HPMA. Polyelectrolyte complexes of small interfering RNAs (siRNA) and the cationic block copolymers were then formed at an N/P ratio between 1 and 4 depending on the block length of the copolymers. Increasing the length of the hydrophilic block was found to decrease the efficiency of siRNA complexation. The hydrodynamic diameter of the polyplexes in 130 mM buffer solution was less than 100 nm.
Original languageEnglish
Pages (from-to)1344 - 1350
Number of pages7
JournalAustralian Journal of Chemistry
Volume62
Issue number10
DOIs
Publication statusPublished - 2009
Externally publishedYes

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