Synthesis of polymers and nanoparticles bearing polystyrene sulfonate brushes for chemokine binding

Naatasha Isahak, Julie Sanchez, Sebastien Perrier, Martin J. Stone, Richard J. Payne

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

The movement of leukocytes to the site of inflammation in response to injury or infection is orchestrated by chemokines binding and signaling through cognate receptors. The interaction between sulfated tyrosine residues on the flexible N-terminal tail of the receptor with positively charged regions of the chemokine is one of the key recognition features that facilitates binding. In this manuscript we describe the synthesis of polymers and silica nanoparticles bearing polystyrene sulfonate brushes to mimic the sulfated tyrosine residues. We show that both the polymers and nanoparticles possess high binding affinity for the chemokine monocyte chemoattractant protein-1 (MCP-1) in monomeric and dimeric form. We also demonstrate key differences in the relative affinity for the chemokine for the free polymer versus the polymer-derived nanoparticle system.
Original languageEnglish
Pages (from-to)5652-5658
Number of pages7
JournalOrganic and Biomolecular Chemistry
Volume14
Issue number24
DOIs
Publication statusPublished - 28 Jun 2016

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