Synthesis, colloidal stability and 64Cu labeling of iron oxide nanoparticles bearing different macrocyclic ligands

Jose Alejandro Barreto Solano, Madlen Matterna, Bimbil Graham, Holger Stephan, Leone Spiccia

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The synthesis, solution stability and 64Cu2+ labeling of magnetite nanoparticles (NPs) coated with different macrocycles is reported, together with the stability of the resulting radioisotope-labeled NPs to transchelation by the competing ligand cyclam, and their stability in blood serum. Three macrocycles, 1,4-bis(2-pyridylmethyl)-1,4,7-triazacyclononane (dmptacn), 1,4,8,11-tetraazacyclotetradecane (cyclam) and 1,4,7,10-tetraazacyclododecane (cyclen), and 3-aminopropyltriethoxysilane were used to modify the magnetite NPs. The ligands were covalently linked to the surface of the NPs with high efficiency by reaction of the corresponding 3-(3-(triethoxysiloxy)propoxy)propan-2-ol derivatives with the NPs. According to transmission electron microscopy (TEM), the uncoated magnetite NPs and macrocycle-functionalized congeners have an average diameter of 6 to 7 nm. The NPs form stable colloidal suspensions in 0.05 M aqueous 2-(N-morpholino)ethanesulfonic acid (MES) buffer, which consist of larger aggregates with a mean hydrodynamic size of about 200 nm. The NPs with the appended macrocycles can be efficiently labeled with 64Cu2+ ions and the radioactivity persists in rat plasma for at least 24 h. Challenge experiments with cyclam also indicate that the radiocopper complexes are highly stable, with the dmptacn-functionalized NPs showing the highest resistance to metal ion leakage. Overall, the dmptacn-functionalized iron oxide NPs provide an excellent platform for the development of robust multimodal cancer imaging/therapeutic agents.
Original languageEnglish
Pages (from-to)2705 - 2712
Number of pages8
JournalNew Journal of Chemistry
Volume35
Issue number11
DOIs
Publication statusPublished - 2011

Cite this

Barreto Solano, Jose Alejandro ; Matterna, Madlen ; Graham, Bimbil ; Stephan, Holger ; Spiccia, Leone. / Synthesis, colloidal stability and 64Cu labeling of iron oxide nanoparticles bearing different macrocyclic ligands. In: New Journal of Chemistry. 2011 ; Vol. 35, No. 11. pp. 2705 - 2712.
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title = "Synthesis, colloidal stability and 64Cu labeling of iron oxide nanoparticles bearing different macrocyclic ligands",
abstract = "The synthesis, solution stability and 64Cu2+ labeling of magnetite nanoparticles (NPs) coated with different macrocycles is reported, together with the stability of the resulting radioisotope-labeled NPs to transchelation by the competing ligand cyclam, and their stability in blood serum. Three macrocycles, 1,4-bis(2-pyridylmethyl)-1,4,7-triazacyclononane (dmptacn), 1,4,8,11-tetraazacyclotetradecane (cyclam) and 1,4,7,10-tetraazacyclododecane (cyclen), and 3-aminopropyltriethoxysilane were used to modify the magnetite NPs. The ligands were covalently linked to the surface of the NPs with high efficiency by reaction of the corresponding 3-(3-(triethoxysiloxy)propoxy)propan-2-ol derivatives with the NPs. According to transmission electron microscopy (TEM), the uncoated magnetite NPs and macrocycle-functionalized congeners have an average diameter of 6 to 7 nm. The NPs form stable colloidal suspensions in 0.05 M aqueous 2-(N-morpholino)ethanesulfonic acid (MES) buffer, which consist of larger aggregates with a mean hydrodynamic size of about 200 nm. The NPs with the appended macrocycles can be efficiently labeled with 64Cu2+ ions and the radioactivity persists in rat plasma for at least 24 h. Challenge experiments with cyclam also indicate that the radiocopper complexes are highly stable, with the dmptacn-functionalized NPs showing the highest resistance to metal ion leakage. Overall, the dmptacn-functionalized iron oxide NPs provide an excellent platform for the development of robust multimodal cancer imaging/therapeutic agents.",
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Synthesis, colloidal stability and 64Cu labeling of iron oxide nanoparticles bearing different macrocyclic ligands. / Barreto Solano, Jose Alejandro; Matterna, Madlen; Graham, Bimbil; Stephan, Holger; Spiccia, Leone.

In: New Journal of Chemistry, Vol. 35, No. 11, 2011, p. 2705 - 2712.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Synthesis, colloidal stability and 64Cu labeling of iron oxide nanoparticles bearing different macrocyclic ligands

AU - Barreto Solano, Jose Alejandro

AU - Matterna, Madlen

AU - Graham, Bimbil

AU - Stephan, Holger

AU - Spiccia, Leone

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AB - The synthesis, solution stability and 64Cu2+ labeling of magnetite nanoparticles (NPs) coated with different macrocycles is reported, together with the stability of the resulting radioisotope-labeled NPs to transchelation by the competing ligand cyclam, and their stability in blood serum. Three macrocycles, 1,4-bis(2-pyridylmethyl)-1,4,7-triazacyclononane (dmptacn), 1,4,8,11-tetraazacyclotetradecane (cyclam) and 1,4,7,10-tetraazacyclododecane (cyclen), and 3-aminopropyltriethoxysilane were used to modify the magnetite NPs. The ligands were covalently linked to the surface of the NPs with high efficiency by reaction of the corresponding 3-(3-(triethoxysiloxy)propoxy)propan-2-ol derivatives with the NPs. According to transmission electron microscopy (TEM), the uncoated magnetite NPs and macrocycle-functionalized congeners have an average diameter of 6 to 7 nm. The NPs form stable colloidal suspensions in 0.05 M aqueous 2-(N-morpholino)ethanesulfonic acid (MES) buffer, which consist of larger aggregates with a mean hydrodynamic size of about 200 nm. The NPs with the appended macrocycles can be efficiently labeled with 64Cu2+ ions and the radioactivity persists in rat plasma for at least 24 h. Challenge experiments with cyclam also indicate that the radiocopper complexes are highly stable, with the dmptacn-functionalized NPs showing the highest resistance to metal ion leakage. Overall, the dmptacn-functionalized iron oxide NPs provide an excellent platform for the development of robust multimodal cancer imaging/therapeutic agents.

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