Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers

Kevin J. Landmark; Stassi DiMaggio; Jesse Ward; Christopher Kelly; Stefan Vogt; Seungpyo Hong; Alina Kotlyar; Andrzej Myc; Thommey P. Thomas; James E. Penner-Hahn; James R. Baker; Mark M. Banaszak Holl; Bradford G. Orr

doi:10.1021/nn800034w

Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers

Kevin J. Landmark, Stassi DiMaggio, Jesse Ward, Christopher Kelly, Stefan Vogt, Seungpyo Hong, Alina Kotlyar, Andrzej Myc, Thommey P. Thomas, James E. Penner-Hahn, James R. Baker, Mark M. Banaszak Holl, Bradford G. Orr

Research output: Contribution to journal › Article › Research › peer-review

166 Citations (Scopus)

Abstract

Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.

Original language	English
Pages (from-to)	773-783
Number of pages	11
Journal	ACS Nano
Volume	2
Issue number	4
DOIs	https://doi.org/10.1021/nn800034w
Publication status	Published - 1 Apr 2008
Externally published	Yes

Keywords

Cancer
Dendrimers
Magnetic nanoparticles
Phase transfer
Superparamagnetism
Targeted MRI contrast agents
X-ray fluorescence microscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1021/nn800034w

Cite this

Landmark, K. J., DiMaggio, S., Ward, J., Kelly, C., Vogt, S., Hong, S., Kotlyar, A., Myc, A., Thomas, T. P., Penner-Hahn, J. E., Baker, J. R., Banaszak Holl, M. M., & Orr, B. G. (2008). Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers. ACS Nano, 2(4), 773-783. https://doi.org/10.1021/nn800034w

@article{2385e74cff0245eda10d68c2da180f20,

title = "Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers",

abstract = "Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.",

keywords = "Cancer, Dendrimers, Magnetic nanoparticles, Phase transfer, Superparamagnetism, Targeted MRI contrast agents, X-ray fluorescence microscopy",

author = "Landmark, {Kevin J.} and Stassi DiMaggio and Jesse Ward and Christopher Kelly and Stefan Vogt and Seungpyo Hong and Alina Kotlyar and Andrzej Myc and Thomas, {Thommey P.} and Penner-Hahn, {James E.} and Baker, {James R.} and {Banaszak Holl}, {Mark M.} and Orr, {Bradford G.}",

year = "2008",

month = apr,

day = "1",

doi = "10.1021/nn800034w",

language = "English",

volume = "2",

pages = "773--783",

journal = "ACS Nano",

issn = "1936-0851",

publisher = "American Chemical Society",

number = "4",

}

Landmark, KJ, DiMaggio, S, Ward, J, Kelly, C, Vogt, S, Hong, S, Kotlyar, A, Myc, A, Thomas, TP, Penner-Hahn, JE, Baker, JR, Banaszak Holl, MM & Orr, BG 2008, 'Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers', ACS Nano, vol. 2, no. 4, pp. 773-783. https://doi.org/10.1021/nn800034w

TY - JOUR

T1 - Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers

AU - Landmark, Kevin J.

AU - DiMaggio, Stassi

AU - Ward, Jesse

AU - Kelly, Christopher

AU - Vogt, Stefan

AU - Hong, Seungpyo

AU - Kotlyar, Alina

AU - Myc, Andrzej

AU - Thomas, Thommey P.

AU - Penner-Hahn, James E.

AU - Baker, James R.

AU - Banaszak Holl, Mark M.

AU - Orr, Bradford G.

PY - 2008/4/1

Y1 - 2008/4/1

N2 - Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.

AB - Organic-coated superparamagnetic iron oxide nanoparticles (OC-SPIONs) were synthesized and characterized by transmission electron microscopy and X-ray photoelectron spectroscopy. OC-SPIONs were transferred from organic media into water using poly(amidoamine) dendrimers modified with 6-TAMRA fluorescent dye and folic acid molecules. The saturation magnetization of the resulting dendrimer-coated SPIONs (DC-SPIONs) was determined, using a superconducting quantum interference device, to be 60 emu/g Fe versus 90 emu/g Fe for bulk magnetite. Selective targeting of the DC-SPIONs to KB cancer cells in vitro was demonstrated and quantified using two distinct and complementary imaging modalities: UV-visible and X-ray fluorescence; confocal microscopy confirmed internalization. The results were consistent between the uptake distribution quantified by flow cytometry using 6-TAMRA UV-visible fluorescence intensity and the cellular iron content determined using X-ray fluorescence microscopy.

KW - Cancer

KW - Dendrimers

KW - Magnetic nanoparticles

KW - Phase transfer

KW - Superparamagnetism

KW - Targeted MRI contrast agents

KW - X-ray fluorescence microscopy

UR - http://www.scopus.com/inward/record.url?scp=46849090307&partnerID=8YFLogxK

U2 - 10.1021/nn800034w

DO - 10.1021/nn800034w

M3 - Article

C2 - 19206610

AN - SCOPUS:46849090307

SN - 1936-0851

VL - 2

SP - 773

EP - 783

JO - ACS Nano

JF - ACS Nano

IS - 4

ER -

Synthesis, characterization, and in vitro testing of superparamagnetic iron oxide nanoparticles targeted using folic acid-conjugated dendrimers

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Cite this