Synthesis and in vitro properties of iron oxide nanoparticles grafted with brushed phosphorylcholine and polyethylene glycol

Thomas Blin, Aleksandr Kakinen, Emily Helen Pilkington, Angela Ivask, Feng Ding, John Quinn, Michael Raymond Whittaker, Pu Chun Ke, Thomas Paul Davis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Polyethylene glycol (PEG) is an established grafting agent for engineered materials deployed in aqueous environments including biological systems. Phosphorylcholine (PC) has shown promise as a viable strategy for enhancing the biofunctionality of surfaces and structures. Here we developed a new and facile strategy for grafting superparamagnetic iron oxide nanoparticles (IONPs) by phosphonic acid terminated poly(2-(methacryloyloxy)ethyl phosphorylcholine) brushes, synthetized by reversible addition-fragmentation chain transfer (RAFT) polymerization. Properties of covalently bound IONPs with PC, PEG or PEG:PC brush-like structures via a "grafting onto" approach through robust bidentate Fe-O-P bonds were compared. The presence of modified polymers on the functionalized IONP surfaces was proved using both ATR-FTIR and TGA. The resultant synthesized IONPs were characterized for their physicochemical and biological aspects. Interestingly, compared with PEG combs, specifically, PC brushes rendered comparable or enhanced suspendability, stability, biocompatibility and cellular distribution. We attribute these characteristics to the biomimetic nature and larger polarity of PC in contrast to the synthetic and hydrophilic PEG. These synthesis strategies and characterizations may prove beneficial to the design and applications of IONPs in nanobiotechnology and nanomedicine.
Original languageEnglish
Pages (from-to)1931-1944
Number of pages14
JournalPolymer Chemistry
Volume7
Issue number10
DOIs
Publication statusPublished - 2016

Cite this

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title = "Synthesis and in vitro properties of iron oxide nanoparticles grafted with brushed phosphorylcholine and polyethylene glycol",
abstract = "Polyethylene glycol (PEG) is an established grafting agent for engineered materials deployed in aqueous environments including biological systems. Phosphorylcholine (PC) has shown promise as a viable strategy for enhancing the biofunctionality of surfaces and structures. Here we developed a new and facile strategy for grafting superparamagnetic iron oxide nanoparticles (IONPs) by phosphonic acid terminated poly(2-(methacryloyloxy)ethyl phosphorylcholine) brushes, synthetized by reversible addition-fragmentation chain transfer (RAFT) polymerization. Properties of covalently bound IONPs with PC, PEG or PEG:PC brush-like structures via a {"}grafting onto{"} approach through robust bidentate Fe-O-P bonds were compared. The presence of modified polymers on the functionalized IONP surfaces was proved using both ATR-FTIR and TGA. The resultant synthesized IONPs were characterized for their physicochemical and biological aspects. Interestingly, compared with PEG combs, specifically, PC brushes rendered comparable or enhanced suspendability, stability, biocompatibility and cellular distribution. We attribute these characteristics to the biomimetic nature and larger polarity of PC in contrast to the synthetic and hydrophilic PEG. These synthesis strategies and characterizations may prove beneficial to the design and applications of IONPs in nanobiotechnology and nanomedicine.",
author = "Thomas Blin and Aleksandr Kakinen and Pilkington, {Emily Helen} and Angela Ivask and Feng Ding and John Quinn and Whittaker, {Michael Raymond} and Ke, {Pu Chun} and Davis, {Thomas Paul}",
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Synthesis and in vitro properties of iron oxide nanoparticles grafted with brushed phosphorylcholine and polyethylene glycol. / Blin, Thomas; Kakinen, Aleksandr; Pilkington, Emily Helen; Ivask, Angela; Ding, Feng; Quinn, John; Whittaker, Michael Raymond; Ke, Pu Chun; Davis, Thomas Paul.

In: Polymer Chemistry, Vol. 7, No. 10, 2016, p. 1931-1944.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Synthesis and in vitro properties of iron oxide nanoparticles grafted with brushed phosphorylcholine and polyethylene glycol

AU - Blin, Thomas

AU - Kakinen, Aleksandr

AU - Pilkington, Emily Helen

AU - Ivask, Angela

AU - Ding, Feng

AU - Quinn, John

AU - Whittaker, Michael Raymond

AU - Ke, Pu Chun

AU - Davis, Thomas Paul

PY - 2016

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N2 - Polyethylene glycol (PEG) is an established grafting agent for engineered materials deployed in aqueous environments including biological systems. Phosphorylcholine (PC) has shown promise as a viable strategy for enhancing the biofunctionality of surfaces and structures. Here we developed a new and facile strategy for grafting superparamagnetic iron oxide nanoparticles (IONPs) by phosphonic acid terminated poly(2-(methacryloyloxy)ethyl phosphorylcholine) brushes, synthetized by reversible addition-fragmentation chain transfer (RAFT) polymerization. Properties of covalently bound IONPs with PC, PEG or PEG:PC brush-like structures via a "grafting onto" approach through robust bidentate Fe-O-P bonds were compared. The presence of modified polymers on the functionalized IONP surfaces was proved using both ATR-FTIR and TGA. The resultant synthesized IONPs were characterized for their physicochemical and biological aspects. Interestingly, compared with PEG combs, specifically, PC brushes rendered comparable or enhanced suspendability, stability, biocompatibility and cellular distribution. We attribute these characteristics to the biomimetic nature and larger polarity of PC in contrast to the synthetic and hydrophilic PEG. These synthesis strategies and characterizations may prove beneficial to the design and applications of IONPs in nanobiotechnology and nanomedicine.

AB - Polyethylene glycol (PEG) is an established grafting agent for engineered materials deployed in aqueous environments including biological systems. Phosphorylcholine (PC) has shown promise as a viable strategy for enhancing the biofunctionality of surfaces and structures. Here we developed a new and facile strategy for grafting superparamagnetic iron oxide nanoparticles (IONPs) by phosphonic acid terminated poly(2-(methacryloyloxy)ethyl phosphorylcholine) brushes, synthetized by reversible addition-fragmentation chain transfer (RAFT) polymerization. Properties of covalently bound IONPs with PC, PEG or PEG:PC brush-like structures via a "grafting onto" approach through robust bidentate Fe-O-P bonds were compared. The presence of modified polymers on the functionalized IONP surfaces was proved using both ATR-FTIR and TGA. The resultant synthesized IONPs were characterized for their physicochemical and biological aspects. Interestingly, compared with PEG combs, specifically, PC brushes rendered comparable or enhanced suspendability, stability, biocompatibility and cellular distribution. We attribute these characteristics to the biomimetic nature and larger polarity of PC in contrast to the synthetic and hydrophilic PEG. These synthesis strategies and characterizations may prove beneficial to the design and applications of IONPs in nanobiotechnology and nanomedicine.

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