TY - JOUR
T1 - Synthesis and Breakdown of pppA2′p5′A2′p5′A and Transient Inhibition of Protein Synthesis in Extracts from Interferon‐Treated and Control Cells
AU - Williams, Bryan R.G.
AU - Kerr, Ian M.
AU - Gilbert, Christopher S.
AU - White, Carol N.
AU - Ball, L. Andrew
PY - 1978/12
Y1 - 1978/12
N2 - Incubation of the mouse l‐cell‐free system with a concentration of pppA2′p5′A2′p5′A [(2′‐5′)An] just sufficient to inhibit protein synthesis results in formation of a high‐molecular‐weight, heatlabile inhibitor and enhanced ribonuclease activity and in the rapid breakdown of (2′‐5′)An to ATP. The (2′‐5′)An‐enhanced ribonuclease activity is also unstable and in the absence of a (2′‐5′)An‐regenerating system inhibition of protein synthesis is transient. Although interferon treatment enhances the synthesis of (2′‐5′)An, the rates of degradation of (2′‐5′)An and levels of activatible nuclease are similar in extracts prepared from control or interferon‐treated cells. Interestingly, the sensitivity of different cell‐free systems to (2′‐5′)An varies with the source of the cell‐free systems and with the methods used in their preparation. There is, however, no obvious correlation between the sensitivities of the system and the rate of breakdown of (2′‐5′)An. The significance of these results is discussed in relation to a possible control function for the (2′‐5′)An system in both interferontreated and control cells.
AB - Incubation of the mouse l‐cell‐free system with a concentration of pppA2′p5′A2′p5′A [(2′‐5′)An] just sufficient to inhibit protein synthesis results in formation of a high‐molecular‐weight, heatlabile inhibitor and enhanced ribonuclease activity and in the rapid breakdown of (2′‐5′)An to ATP. The (2′‐5′)An‐enhanced ribonuclease activity is also unstable and in the absence of a (2′‐5′)An‐regenerating system inhibition of protein synthesis is transient. Although interferon treatment enhances the synthesis of (2′‐5′)An, the rates of degradation of (2′‐5′)An and levels of activatible nuclease are similar in extracts prepared from control or interferon‐treated cells. Interestingly, the sensitivity of different cell‐free systems to (2′‐5′)An varies with the source of the cell‐free systems and with the methods used in their preparation. There is, however, no obvious correlation between the sensitivities of the system and the rate of breakdown of (2′‐5′)An. The significance of these results is discussed in relation to a possible control function for the (2′‐5′)An system in both interferontreated and control cells.
UR - http://www.scopus.com/inward/record.url?scp=0018215440&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1978.tb12767.x
DO - 10.1111/j.1432-1033.1978.tb12767.x
M3 - Article
C2 - 216547
AN - SCOPUS:0018215440
SN - 0014-2956
VL - 92
SP - 455
EP - 462
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 2
ER -