Synthesis and bioactivity of poly(HPMA)-lysozyme conjugates: The use of novel thiazolidine-2-thione coupling chemistry

Lei Tao, Jingquan Liu, Jiangtao Xu, Thomas Paul Davis

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77 Citations (Scopus)

Abstract

A novel thiazolidine-2-thione functionalized chain transfer agent (CTA) was synthesized and used as a reversible addition-fragmentation chain transfer (RAFT) polymerization agent to prepare well-defined poly-N-(2-hydroxypropyl) methacrylamide (PHPMA). The polymer chains had pre-designed molecular weights, narrow polydispersities and were chain-end functionalized. On incubation with protein (lysozyme) under different pH conditions, PHPMA was conjugated to the protein surface via covalent amide bonding. The bioactivity of the lysozyme-PHPMA conjugates was assessed using Micrococcus lysodeikticus (Ml) cells as substrates. The number of polymer chains attached to the protein could be controlled by both the pH of the conjugation reaction and the molecular weights of the polymers, thereby influencing significantly the bioactivity of the protein-polymer conjugates.
Original languageEnglish
Pages (from-to)3481 - 3485
Number of pages5
JournalOrganic and Biomolecular Chemistry
Volume7
Issue number17
DOIs
Publication statusPublished - 2009
Externally publishedYes

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