Synergistic toxicity of copper and gold compounds in Cupriavidus metallidurans

Nicole Wiesemann, Lucy Bütof, Martin Herzberg, Gerd Hause, Lutz Berthold, Barbara Etschmann, Joël Brugger, Gema Martinez-Criado, Dirk Dobritzsch, Sacha Baginsky, Frank Reith, Dietrich H. Nies

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Abstract

The bacterium Cupriavidus metallidurans can reduce toxic gold(I/III) complexes and biomineralize them into metallic gold (Au) nanoparticles, thereby mediating the (trans)formation of Au nuggets. In Au-rich soils, most transition metals do not interfere with the resistance of this bacterium to toxic mobile Au complexes and can be removed from the cell by plasmid-encoded metal efflux systems. Copper is a noticeable exception: the presence of Au complexes and Cu ions results in synergistic toxicity, which is accompanied by an increased cytoplasmic Cu content and formation of Au nanoparticles in the periplasm. The periplasmic Cu-oxidase CopA was not essential for formation of the periplasmic Au nanoparticles. As shown with the purified and reconstituted Cu efflux system CupA, Au complexes block Cu dependent release of phosphate from ATP by CupA, indicating inhibition of Cu transport. Moreover, Cu resistance of Au-inhibited cells was similar to that of mutants carrying deletions in the genes for the Cu-exporting PIB1-type ATPases. Consequently, Au complexes inhibit export of cytoplasmic Cu ions, leading to an increased cellular Cu content and decreased Cu and Au resistance. Uncovering the biochemical mechanisms of synergistic Au and Cu toxicity in C. metallidurans explains the issues this bacterium has to face in auriferous environments, where it is an important contributor to the environmental Au cycle.

Original languageEnglish
Article numbere01679-17
Number of pages17
JournalApplied and Environmental Microbiology
Volume83
Issue number23
DOIs
Publication statusPublished - 1 Jan 2017

Keywords

  • Biomineralization
  • Copper
  • Cu-dependent oxidase
  • Gold
  • Toxicity

Cite this

Wiesemann, N., Bütof, L., Herzberg, M., Hause, G., Berthold, L., Etschmann, B., Brugger, J., Martinez-Criado, G., Dobritzsch, D., Baginsky, S., Reith, F., & Nies, D. H. (2017). Synergistic toxicity of copper and gold compounds in Cupriavidus metallidurans. Applied and Environmental Microbiology, 83(23), [e01679-17]. https://doi.org/10.1128/AEM.01679-17