Synaptic binding sites in brain for [3H]β-bungarotoxin - A specific probe that perturbs transmitter release

Iekhsan B. Othman, Graham P. Wilkin, J. Oliver Dolly

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A neurotoxic, [3H]labelled derivative of β-bungarotoxin, known to inhibit neurotransmitter release and to be free of phospholipase activity, was used to demonstrate autoradiographically the distribution and ultrastructural location of its saturable binding component in brain. Light-microscope autoradiography of rat cerebellum and hippocampus showed that it resides primarily in synaptic-rich areas, with much lower densities of sites in other regions containing cell bodies; also, little binding was associated with myelinated tracts. Ultrastructural localisation and sub-fractionation studies on purified cerebrocortical synaptosomes showed that [3H]toxin binding sites are located predominantly on brain synaptosomal membranes, consistent with their possible association with transmitter release.

Original languageEnglish
Pages (from-to)487-496
Number of pages10
JournalNeurochemistry International
Issue number4
Publication statusPublished - 1983
Externally publishedYes

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