Sympathetic neural activation does not mediate heart rate variability during repeated brief umbilical cord occlusions in near-term fetal sheep

Christopher A. Lear, Robert Galinsky, Guido Wassink, Clinton J. Mitchell, Joanne O. Davidson, Jennifer A. Westgate, Laura Bennet, Alistair J. Gunn

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Changes in fetal heart rate variability (FHRV) and ST segment elevation (measured as the T/QRS ratio) are used to evaluate fetal adaptation to labour. The sympathetic nervous system (SNS) is an important contributor to FHRV under healthy normoxic conditions, and is critical for rapid support of blood pressure during brief labour-like asphyxia. However, although it has been assumed that SNS activity contributes to FHRV during labour; this has never been tested, and it is unclear whether the SNS contributes to the rapid increase in T/QRS ratio during brief asphyxia. Thirteen chronically instrumented fetal sheep at 0.85 of gestation received either chemical sympathectomy with 6-hydroxydopamine (6-OHDA; n = 6) or sham treatment (control; n = 7), followed 4-5 days later by 2 min episodes of complete umbilical cord occlusion repeated every 5 min for up to 4 h, or until mean arterial blood pressure fell to <20 mmHg for two successive occlusions. FHRV was decreased before occlusions in the 6-OHDA group (P < 0.05) and 2-4.5 h during recovery after occlusions (P < 0.05) compared to the control group. During each occlusion there was a rapid increase in T/QRS ratio. Between successive occlusions the T/QRS ratio rapidly returned to baseline, and FHRV increased above baseline in both groups (P < 0.05), with no significant effect of sympathectomy on FHRV or T/QRS ratio. In conclusion, these data show that SNS activity does not mediate the increase in FHRV between repeated episodes of brief umbilical cord occlusion or the transient increase in T/QRS ratio during occlusions.

Original languageEnglish
Pages (from-to)1265-1277
Number of pages13
JournalJournal of Physiology
Volume594
Issue number5
DOIs
Publication statusPublished - 1 Mar 2016
Externally publishedYes

Cite this

Lear, Christopher A. ; Galinsky, Robert ; Wassink, Guido ; Mitchell, Clinton J. ; Davidson, Joanne O. ; Westgate, Jennifer A. ; Bennet, Laura ; Gunn, Alistair J. / Sympathetic neural activation does not mediate heart rate variability during repeated brief umbilical cord occlusions in near-term fetal sheep. In: Journal of Physiology. 2016 ; Vol. 594, No. 5. pp. 1265-1277.
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abstract = "Changes in fetal heart rate variability (FHRV) and ST segment elevation (measured as the T/QRS ratio) are used to evaluate fetal adaptation to labour. The sympathetic nervous system (SNS) is an important contributor to FHRV under healthy normoxic conditions, and is critical for rapid support of blood pressure during brief labour-like asphyxia. However, although it has been assumed that SNS activity contributes to FHRV during labour; this has never been tested, and it is unclear whether the SNS contributes to the rapid increase in T/QRS ratio during brief asphyxia. Thirteen chronically instrumented fetal sheep at 0.85 of gestation received either chemical sympathectomy with 6-hydroxydopamine (6-OHDA; n = 6) or sham treatment (control; n = 7), followed 4-5 days later by 2 min episodes of complete umbilical cord occlusion repeated every 5 min for up to 4 h, or until mean arterial blood pressure fell to <20 mmHg for two successive occlusions. FHRV was decreased before occlusions in the 6-OHDA group (P < 0.05) and 2-4.5 h during recovery after occlusions (P < 0.05) compared to the control group. During each occlusion there was a rapid increase in T/QRS ratio. Between successive occlusions the T/QRS ratio rapidly returned to baseline, and FHRV increased above baseline in both groups (P < 0.05), with no significant effect of sympathectomy on FHRV or T/QRS ratio. In conclusion, these data show that SNS activity does not mediate the increase in FHRV between repeated episodes of brief umbilical cord occlusion or the transient increase in T/QRS ratio during occlusions.",
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Sympathetic neural activation does not mediate heart rate variability during repeated brief umbilical cord occlusions in near-term fetal sheep. / Lear, Christopher A.; Galinsky, Robert; Wassink, Guido; Mitchell, Clinton J.; Davidson, Joanne O.; Westgate, Jennifer A.; Bennet, Laura; Gunn, Alistair J.

In: Journal of Physiology, Vol. 594, No. 5, 01.03.2016, p. 1265-1277.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Sympathetic neural activation does not mediate heart rate variability during repeated brief umbilical cord occlusions in near-term fetal sheep

AU - Lear, Christopher A.

AU - Galinsky, Robert

AU - Wassink, Guido

AU - Mitchell, Clinton J.

AU - Davidson, Joanne O.

AU - Westgate, Jennifer A.

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AU - Gunn, Alistair J.

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