Suspended in culture - human pluripotent cells for scalable technologies

Carmel O'Brien, Andrew L Laslett

Research output: Contribution to journalLetterOther

16 Citations (Scopus)


Human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), collectively termed human pluripotent stem cells (hPSCs), are typically derived and maintained in adherent and semi-defined culture conditions. Recently a number of groups, including Chen et al., 2012, have demonstrated that hESCs can now be expanded efficiently and maintain pluripotency over long-term passaging as aggregates in a serum-free defined suspension culture system, permitting the preparation of scalable cGMP derived hPSC cultures for cell banking, high throughput research programs and clinical applications. In this short commentary we describe the utility and potential future uses of suspension culture systems for hPSCs.
Original languageEnglish
Pages (from-to)167 - 170
Number of pages4
JournalStem Cell Research
Issue number2
Publication statusPublished - 2012

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