TY - JOUR
T1 - Surface plasmon resonance assay for chloramphenicol without surface regeneration
AU - Yuan, Jing
AU - Addo, James
AU - Aguilar, Marie Isabel
AU - Wu, Yinqiu
PY - 2009/7/1
Y1 - 2009/7/1
N2 - A surface plasmon resonance (SPR) assay without surface regeneration was developed for rapid and sensitive detection of chloramphenicol (CAP). A CAP-amine derivative was synthesized using a polyethylene glycol chain attached to the CAP through a carbamate linkage and immobilized onto a Biacore dextran surface. This chemically modified surface significantly changed the binding behavior between antibody and CAP, shown by both fast association and fast dissociation rates, and created a rapid and sensitive SPR immunoassay of the CAP without any regeneration. The limits of detection achieved for CAP were 32.2 pg/ml in aqueous buffer and 42.4 pg/ml in honey-spiked samples.
AB - A surface plasmon resonance (SPR) assay without surface regeneration was developed for rapid and sensitive detection of chloramphenicol (CAP). A CAP-amine derivative was synthesized using a polyethylene glycol chain attached to the CAP through a carbamate linkage and immobilized onto a Biacore dextran surface. This chemically modified surface significantly changed the binding behavior between antibody and CAP, shown by both fast association and fast dissociation rates, and created a rapid and sensitive SPR immunoassay of the CAP without any regeneration. The limits of detection achieved for CAP were 32.2 pg/ml in aqueous buffer and 42.4 pg/ml in honey-spiked samples.
UR - http://www.scopus.com/inward/record.url?scp=67349152078&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.04.003
DO - 10.1016/j.ab.2009.04.003
M3 - Article
C2 - 19358818
AN - SCOPUS:67349152078
VL - 390
SP - 97
EP - 99
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -