Surface plasmon resonance assay for chloramphenicol without surface regeneration

Jing Yuan, James Addo, Marie Isabel Aguilar, Yinqiu Wu

Research output: Contribution to journalArticleOtherpeer-review

21 Citations (Scopus)

Abstract

A surface plasmon resonance (SPR) assay without surface regeneration was developed for rapid and sensitive detection of chloramphenicol (CAP). A CAP-amine derivative was synthesized using a polyethylene glycol chain attached to the CAP through a carbamate linkage and immobilized onto a Biacore dextran surface. This chemically modified surface significantly changed the binding behavior between antibody and CAP, shown by both fast association and fast dissociation rates, and created a rapid and sensitive SPR immunoassay of the CAP without any regeneration. The limits of detection achieved for CAP were 32.2 pg/ml in aqueous buffer and 42.4 pg/ml in honey-spiked samples.

Original languageEnglish
Pages (from-to)97-99
Number of pages3
JournalAnalytical Biochemistry
Volume390
Issue number1
DOIs
Publication statusPublished - 1 Jul 2009
Externally publishedYes

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