Surface physico-chemical studies of immobilised oligonucleotides

P. C.T. Nguyen; S. Kumar; M. Denichilo; N. Voelcker; H. J. Griesser

Surface physico-chemical studies of immobilised oligonucleotides

P. C.T. Nguyen
, S. Kumar
, M. Denichilo
, N. Voelcker
, H. J. Griesser

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Other › peer-review

Abstract

The effects of physical and chemical factors of the substrate chemistry and oligonucleotide chemistry on the immobilization process and efficiency were studied. The oligonucleotides terminated by various functional groups were immobilized to functionalized substrates prepared by plasma polymerization. The surface analysis of the propanol surface, immobilized oligonucleotides and immobilized control were performed using X-ray photoelectron spectroscopy (XPS) and time-of-flight- secondary ion mass spectrometry (TOF-SIMS). The effectiveness of oligonucleotides over antibodies as recognition elements in microarrays for high throughput screening were recognized and applied to medical diagnosis and plant pathogen detection.

Original language	English
Title of host publication	Transactions - 7th World Biomaterials Congress
Number of pages	1
Publication status	Published - 1 Dec 2004
Externally published	Yes
Event	World Biomaterials Congress (WBC) 2004 - Sydney, Australia Duration: 17 May 2004 → 21 May 2004 Conference number: 7th

Conference

Conference	World Biomaterials Congress (WBC) 2004
Country/Territory	Australia
City	Sydney
Period	17/05/04 → 21/05/04

Cite this

@inproceedings{50b884f65f054c37a6f533cfe8253a09,

title = "Surface physico-chemical studies of immobilised oligonucleotides",

abstract = "The effects of physical and chemical factors of the substrate chemistry and oligonucleotide chemistry on the immobilization process and efficiency were studied. The oligonucleotides terminated by various functional groups were immobilized to functionalized substrates prepared by plasma polymerization. The surface analysis of the propanol surface, immobilized oligonucleotides and immobilized control were performed using X-ray photoelectron spectroscopy (XPS) and time-of-flight- secondary ion mass spectrometry (TOF-SIMS). The effectiveness of oligonucleotides over antibodies as recognition elements in microarrays for high throughput screening were recognized and applied to medical diagnosis and plant pathogen detection.",

author = "Nguyen, \{P. C.T.\} and S. Kumar and M. Denichilo and N. Voelcker and Griesser, \{H. J.\}",

year = "2004",

month = dec,

day = "1",

language = "English",

isbn = "1877040193",

booktitle = "Transactions - 7th World Biomaterials Congress",

note = "World Biomaterials Congress (WBC) 2004 ; Conference date: 17-05-2004 Through 21-05-2004",

}

TY - GEN

T1 - Surface physico-chemical studies of immobilised oligonucleotides

AU - Nguyen, P. C.T.

AU - Kumar, S.

AU - Denichilo, M.

AU - Voelcker, N.

AU - Griesser, H. J.

N1 - Conference code: 7th

PY - 2004/12/1

Y1 - 2004/12/1

N2 - The effects of physical and chemical factors of the substrate chemistry and oligonucleotide chemistry on the immobilization process and efficiency were studied. The oligonucleotides terminated by various functional groups were immobilized to functionalized substrates prepared by plasma polymerization. The surface analysis of the propanol surface, immobilized oligonucleotides and immobilized control were performed using X-ray photoelectron spectroscopy (XPS) and time-of-flight- secondary ion mass spectrometry (TOF-SIMS). The effectiveness of oligonucleotides over antibodies as recognition elements in microarrays for high throughput screening were recognized and applied to medical diagnosis and plant pathogen detection.

AB - The effects of physical and chemical factors of the substrate chemistry and oligonucleotide chemistry on the immobilization process and efficiency were studied. The oligonucleotides terminated by various functional groups were immobilized to functionalized substrates prepared by plasma polymerization. The surface analysis of the propanol surface, immobilized oligonucleotides and immobilized control were performed using X-ray photoelectron spectroscopy (XPS) and time-of-flight- secondary ion mass spectrometry (TOF-SIMS). The effectiveness of oligonucleotides over antibodies as recognition elements in microarrays for high throughput screening were recognized and applied to medical diagnosis and plant pathogen detection.

UR - https://www.scopus.com/pages/publications/13844273217

M3 - Conference Paper

AN - SCOPUS:13844273217

SN - 1877040193

SN - 9781877040191

BT - Transactions - 7th World Biomaterials Congress

T2 - World Biomaterials Congress (WBC) 2004

Y2 - 17 May 2004 through 21 May 2004

ER -

Surface physico-chemical studies of immobilised oligonucleotides

Abstract

Conference

Other files and links

Cite this