Surface physico-chemical studies of immobilised oligonucleotides

P. C.T. Nguyen, S. Kumar, M. Denichilo, N. Voelcker, H. J. Griesser

Research output: Chapter in Book/Report/Conference proceedingConference PaperOtherpeer-review

Abstract

The effects of physical and chemical factors of the substrate chemistry and oligonucleotide chemistry on the immobilization process and efficiency were studied. The oligonucleotides terminated by various functional groups were immobilized to functionalized substrates prepared by plasma polymerization. The surface analysis of the propanol surface, immobilized oligonucleotides and immobilized control were performed using X-ray photoelectron spectroscopy (XPS) and time-of-flight- secondary ion mass spectrometry (TOF-SIMS). The effectiveness of oligonucleotides over antibodies as recognition elements in microarrays for high throughput screening were recognized and applied to medical diagnosis and plant pathogen detection.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 1 Dec 2004
Externally publishedYes
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 17 May 200421 May 2004

Publication series

NameTransactions - 7th World Biomaterials Congress

Conference

ConferenceTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period17/05/0421/05/04

Cite this