[3H]nemonapride binding in human caudate and putamen

Phillip L. Marzella, David Copolov

Research output: Contribution to journalArticleResearchpeer-review

5 Citations (Scopus)

Abstract

The binding of [3H]nemonapride to human postmortem caudate and putamen tissue was autoradiographically investigated using several antipsychotic drugs. Saturation experiments revealed a single population of binding sites (dissociation constant (K(D)) 0.38 ± 0.01 nM, and total binding capacity (B(MAX)) 5S fmol/TE). Prototypic dopamine (DA) receptors antagonists displaced [3H]nemonapride in a monophasic manner. The order of displacement potency was expected for DA D2-like receptors: spiperone > (+)butaclamol ≤ chlorpromazine > (-)sulpiride > ketanserin. Displacement with serotonergic antagonists suggests that in human caudate and putamen tissue [3H]nemonapride may have a very low affinity serotonergic component. However, [3H]nemonapride displays a high affinity and selectivity for DA D2-like receptors and should make it a preferred compound for tritium-based autoradiography.

Original languageEnglish
Pages (from-to)167-170
Number of pages4
JournalBrain Research Bulletin
Volume44
Issue number2
DOIs
Publication statusPublished - 1 Jan 1997
Externally publishedYes

Keywords

  • [H]Nemonapride binding
  • Human caudate
  • Human putamen

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