31P Nuclear‐Magnetic‐Resonance Studies of Energy Metabolism in Tissue from the Marine Invertebrate Tapes watlingi

Kevin D. BARROW, Dana D. JAMIESON, Raymond S. NORTON

Research output: Contribution to journalArticleResearchpeer-review

47 Citations (Scopus)

Abstract

31P nuclear magnetic resonance spectra of tissue from the marine bivalve mollusc Tapes watlingi are presented. Spectra from foot muscle, adductor muscle and hearts contain resonances from the adenine nucleotides, inorganic phosphate and a phosphagen which is shown to be arginine phosphate. The intracellular pH of the tissue, measured from the chemical shift of the inorganic phosphate resonance, is 7.2, which is significantly lower than that of sea water, viz. 8.1. The importance of a correct choice of solution conditions for determining a correlation curve of chemical shift versus pH for tissue from marine organisms is emphasized. Spectra of perchloric acid extracts of muscle are used to determine levels of the major phosphorus‐containing metabolites in the tissue. The time courses of changes in the phosphorus‐containing metabolites of foot and adductor muscle are described. Under anaerobic conditions the sequence of events is similar to that observed in vertebrate skeletal muscle, but the overall rate of decay is much slower, e.g. arginine phosphate is present in foot muscle for almost 20 h at its physiological temperature. Oxygenation of the incubation medium prolongs the survival time of the tissue. Treatment with iodoacetamide and sodium azide under anaerobic conditions reduces the lifetime and changes the pattern of decay such that ATP begins to decay before the phosphagen is consumed, and most of the phosphate accumulates in sugar phosphates, mainly fructose 1,6‐biphosphate. The effect of low salinity stress is also examined. Spectra of muscle from animals exposed to 50% and 75% normal sea water for periods of about four weeks are essentially identical with those of normal animals. Furthermore, the decay of intact muscle incubated in 50% sea water is similar to that in normal sea water.

Original languageEnglish
Pages (from-to)289-297
Number of pages9
JournalEuropean Journal of Biochemistry
Volume103
Issue number2
DOIs
Publication statusPublished - 1980
Externally publishedYes

Cite this

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title = "31P Nuclear‐Magnetic‐Resonance Studies of Energy Metabolism in Tissue from the Marine Invertebrate Tapes watlingi",
abstract = "31P nuclear magnetic resonance spectra of tissue from the marine bivalve mollusc Tapes watlingi are presented. Spectra from foot muscle, adductor muscle and hearts contain resonances from the adenine nucleotides, inorganic phosphate and a phosphagen which is shown to be arginine phosphate. The intracellular pH of the tissue, measured from the chemical shift of the inorganic phosphate resonance, is 7.2, which is significantly lower than that of sea water, viz. 8.1. The importance of a correct choice of solution conditions for determining a correlation curve of chemical shift versus pH for tissue from marine organisms is emphasized. Spectra of perchloric acid extracts of muscle are used to determine levels of the major phosphorus‐containing metabolites in the tissue. The time courses of changes in the phosphorus‐containing metabolites of foot and adductor muscle are described. Under anaerobic conditions the sequence of events is similar to that observed in vertebrate skeletal muscle, but the overall rate of decay is much slower, e.g. arginine phosphate is present in foot muscle for almost 20 h at its physiological temperature. Oxygenation of the incubation medium prolongs the survival time of the tissue. Treatment with iodoacetamide and sodium azide under anaerobic conditions reduces the lifetime and changes the pattern of decay such that ATP begins to decay before the phosphagen is consumed, and most of the phosphate accumulates in sugar phosphates, mainly fructose 1,6‐biphosphate. The effect of low salinity stress is also examined. Spectra of muscle from animals exposed to 50{\%} and 75{\%} normal sea water for periods of about four weeks are essentially identical with those of normal animals. Furthermore, the decay of intact muscle incubated in 50{\%} sea water is similar to that in normal sea water.",
author = "BARROW, {Kevin D.} and JAMIESON, {Dana D.} and NORTON, {Raymond S.}",
year = "1980",
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language = "English",
volume = "103",
pages = "289--297",
journal = "European Journal of Biochemistry",
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31P Nuclear‐Magnetic‐Resonance Studies of Energy Metabolism in Tissue from the Marine Invertebrate Tapes watlingi. / BARROW, Kevin D.; JAMIESON, Dana D.; NORTON, Raymond S.

In: European Journal of Biochemistry, Vol. 103, No. 2, 1980, p. 289-297.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - 31P Nuclear‐Magnetic‐Resonance Studies of Energy Metabolism in Tissue from the Marine Invertebrate Tapes watlingi

AU - BARROW, Kevin D.

AU - JAMIESON, Dana D.

AU - NORTON, Raymond S.

PY - 1980

Y1 - 1980

N2 - 31P nuclear magnetic resonance spectra of tissue from the marine bivalve mollusc Tapes watlingi are presented. Spectra from foot muscle, adductor muscle and hearts contain resonances from the adenine nucleotides, inorganic phosphate and a phosphagen which is shown to be arginine phosphate. The intracellular pH of the tissue, measured from the chemical shift of the inorganic phosphate resonance, is 7.2, which is significantly lower than that of sea water, viz. 8.1. The importance of a correct choice of solution conditions for determining a correlation curve of chemical shift versus pH for tissue from marine organisms is emphasized. Spectra of perchloric acid extracts of muscle are used to determine levels of the major phosphorus‐containing metabolites in the tissue. The time courses of changes in the phosphorus‐containing metabolites of foot and adductor muscle are described. Under anaerobic conditions the sequence of events is similar to that observed in vertebrate skeletal muscle, but the overall rate of decay is much slower, e.g. arginine phosphate is present in foot muscle for almost 20 h at its physiological temperature. Oxygenation of the incubation medium prolongs the survival time of the tissue. Treatment with iodoacetamide and sodium azide under anaerobic conditions reduces the lifetime and changes the pattern of decay such that ATP begins to decay before the phosphagen is consumed, and most of the phosphate accumulates in sugar phosphates, mainly fructose 1,6‐biphosphate. The effect of low salinity stress is also examined. Spectra of muscle from animals exposed to 50% and 75% normal sea water for periods of about four weeks are essentially identical with those of normal animals. Furthermore, the decay of intact muscle incubated in 50% sea water is similar to that in normal sea water.

AB - 31P nuclear magnetic resonance spectra of tissue from the marine bivalve mollusc Tapes watlingi are presented. Spectra from foot muscle, adductor muscle and hearts contain resonances from the adenine nucleotides, inorganic phosphate and a phosphagen which is shown to be arginine phosphate. The intracellular pH of the tissue, measured from the chemical shift of the inorganic phosphate resonance, is 7.2, which is significantly lower than that of sea water, viz. 8.1. The importance of a correct choice of solution conditions for determining a correlation curve of chemical shift versus pH for tissue from marine organisms is emphasized. Spectra of perchloric acid extracts of muscle are used to determine levels of the major phosphorus‐containing metabolites in the tissue. The time courses of changes in the phosphorus‐containing metabolites of foot and adductor muscle are described. Under anaerobic conditions the sequence of events is similar to that observed in vertebrate skeletal muscle, but the overall rate of decay is much slower, e.g. arginine phosphate is present in foot muscle for almost 20 h at its physiological temperature. Oxygenation of the incubation medium prolongs the survival time of the tissue. Treatment with iodoacetamide and sodium azide under anaerobic conditions reduces the lifetime and changes the pattern of decay such that ATP begins to decay before the phosphagen is consumed, and most of the phosphate accumulates in sugar phosphates, mainly fructose 1,6‐biphosphate. The effect of low salinity stress is also examined. Spectra of muscle from animals exposed to 50% and 75% normal sea water for periods of about four weeks are essentially identical with those of normal animals. Furthermore, the decay of intact muscle incubated in 50% sea water is similar to that in normal sea water.

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DO - 10.1111/j.1432-1033.1980.tb04314.x

M3 - Article

VL - 103

SP - 289

EP - 297

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

SN - 0014-2956

IS - 2

ER -