Sulphate reducing bacteria (SRB) biofilm development and its role in microbial corrosion of carbon steel

Sachie Ruwanthika Welikala, Saad Al-Saadi, Will P. Gates, Christopher Panter, Raman R.K. Singh

Research output: Contribution to journalArticleResearchpeer-review


The development of biofilm by pure SRB culture on carbon steel, and its role on corrosion were investigated using microscopic, spectroscopic, electrochemical and surface characterization techniques. Tubercle biofilm and irregularly shaped pits were observed on steel surfaces in high-nutrient biotic solution. Owing to development of a protective FeS film in 72 h immersion, corrosion resistance improved. In nutrient-deficient medium, a greater bacterial density attached to the metal surface as the consequence of starved bacteria seeking energy sources from metal. However, electrochemical non-homogeneity developed at the locations of their attachment, that gradually grew over the entire surface.

Original languageEnglish
Article number1360869
Number of pages18
JournalFrontiers in Materials
Publication statusPublished - 20 Feb 2024


  • biofilm
  • environmental scanning electron microscopy (ESEM)
  • focused ion beam-scanning electron microscopy (FIB-SEM)
  • microbial corrosion
  • sulphate reducing bacteria (SRB)

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