Successful in vitro expansion and differentiation of cord blood derived CD34+ cells into early endothelial progenitor cells reveals highly differential gene expression

Ingo Ahrens; Helena Domeij; Denijal Topcic; Izhak Haviv; Ruusu-Maaria Merivirta; Alex Agrotis; Ephraem Leitner; Jeremy B Jowett; Christoph Bode; Martha Lappas; Karlheinz Peter

doi:10.1371/journal.pone.0023210

Successful in vitro expansion and differentiation of cord blood derived CD34+ cells into early endothelial progenitor cells reveals highly differential gene expression

Ingo Ahrens, Helena Domeij, Denijal Topcic, Izhak Haviv, Ruusu-Maaria Merivirta, Alex Agrotis, Ephraem Leitner, Jeremy B Jowett, Christoph Bode, Martha Lappas, Karlheinz Peter

Immunology Alfred Hospital

Research output: Contribution to journal › Article › Research › peer-review

40 Citations (Scopus)

Abstract

Endothelial progenitor cells (EPCs) can be purified from peripheral blood, bone marrow or cord blood and are typically defined by a limited number of cell surface markers and a few functional tests. A detailed in vitro characterization is often restricted by the low cell numbers of circulating EPCs. Therefore in vitro culturing and expansion methods are applied, which allow at least distinguishing two different types of EPCs, early and late EPCs. Herein, we describe an in vitro culture technique with the aim to generate high numbers of phenotypically, functionally and genetically defined early EPCs from human cord blood. Characterization of EPCs was done by flow cytometry, immunofluorescence microscopy, colony forming unit (CFU) assay and endothelial tube formation assay.

Original language	English
Article number	e23210
Number of pages	12
Journal	PLoS ONE
Volume	6
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0023210
Publication status	Published - 2011

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Ahrens, I., Domeij, H., Topcic, D., Haviv, I., Merivirta, R-M., Agrotis, A., Leitner, E., Jowett, J. B., Bode, C., Lappas, M., & Peter, K. (2011). Successful in vitro expansion and differentiation of cord blood derived CD34+ cells into early endothelial progenitor cells reveals highly differential gene expression. PLoS ONE, 6(8), [e23210]. https://doi.org/10.1371/journal.pone.0023210

Ahrens, Ingo ; Domeij, Helena ; Topcic, Denijal ; Haviv, Izhak ; Merivirta, Ruusu-Maaria ; Agrotis, Alex ; Leitner, Ephraem ; Jowett, Jeremy B ; Bode, Christoph ; Lappas, Martha ; Peter, Karlheinz. / Successful in vitro expansion and differentiation of cord blood derived CD34+ cells into early endothelial progenitor cells reveals highly differential gene expression. In: PLoS ONE. 2011 ; Vol. 6, No. 8.

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abstract = "Endothelial progenitor cells (EPCs) can be purified from peripheral blood, bone marrow or cord blood and are typically defined by a limited number of cell surface markers and a few functional tests. A detailed in vitro characterization is often restricted by the low cell numbers of circulating EPCs. Therefore in vitro culturing and expansion methods are applied, which allow at least distinguishing two different types of EPCs, early and late EPCs. Herein, we describe an in vitro culture technique with the aim to generate high numbers of phenotypically, functionally and genetically defined early EPCs from human cord blood. Characterization of EPCs was done by flow cytometry, immunofluorescence microscopy, colony forming unit (CFU) assay and endothelial tube formation assay.",

author = "Ingo Ahrens and Helena Domeij and Denijal Topcic and Izhak Haviv and Ruusu-Maaria Merivirta and Alex Agrotis and Ephraem Leitner and Jowett, {Jeremy B} and Christoph Bode and Martha Lappas and Karlheinz Peter",

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Ahrens, I, Domeij, H, Topcic, D, Haviv, I, Merivirta, R-M, Agrotis, A, Leitner, E, Jowett, JB, Bode, C, Lappas, M & Peter, K 2011, 'Successful in vitro expansion and differentiation of cord blood derived CD34+ cells into early endothelial progenitor cells reveals highly differential gene expression', PLoS ONE, vol. 6, no. 8, e23210. https://doi.org/10.1371/journal.pone.0023210

Successful in vitro expansion and differentiation of cord blood derived CD34+ cells into early endothelial progenitor cells reveals highly differential gene expression. / Ahrens, Ingo; Domeij, Helena; Topcic, Denijal; Haviv, Izhak; Merivirta, Ruusu-Maaria; Agrotis, Alex; Leitner, Ephraem; Jowett, Jeremy B; Bode, Christoph; Lappas, Martha; Peter, Karlheinz.

In: PLoS ONE, Vol. 6, No. 8, e23210, 2011.

Research output: Contribution to journal › Article › Research › peer-review

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AU - Merivirta, Ruusu-Maaria

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AU - Peter, Karlheinz

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