Successful in vitro expansion and differentiation of cord blood derived CD34+ cells into early endothelial progenitor cells reveals highly differential gene expression

Ingo Ahrens, Helena Domeij, Denijal Topcic, Izhak Haviv, Ruusu-Maaria Merivirta, Alex Agrotis, Ephraem Leitner, Jeremy B Jowett, Christoph Bode, Martha Lappas, Karlheinz Peter

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Abstract

Endothelial progenitor cells (EPCs) can be purified from peripheral blood, bone marrow or cord blood and are typically defined by a limited number of cell surface markers and a few functional tests. A detailed in vitro characterization is often restricted by the low cell numbers of circulating EPCs. Therefore in vitro culturing and expansion methods are applied, which allow at least distinguishing two different types of EPCs, early and late EPCs. Herein, we describe an in vitro culture technique with the aim to generate high numbers of phenotypically, functionally and genetically defined early EPCs from human cord blood. Characterization of EPCs was done by flow cytometry, immunofluorescence microscopy, colony forming unit (CFU) assay and endothelial tube formation assay.
Original languageEnglish
Article numbere23210
Number of pages12
JournalPLoS ONE
Volume6
Issue number8
DOIs
Publication statusPublished - 2011

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