Subtyping intimin genes from enteropathogenic Escherichia coli associated with outbreaks and sporadic cases in the United Kingdom and Eire

C. Jenkins, A. J. Lawson, T. Cheasty, G. A. Willshaw, P. Wright, G. Dougan, G. Frankel, H. R. Smith

Research output: Contribution to journalArticleResearchpeer-review

48 Citations (Scopus)


PCR-RFLP methods for subtyping the intimin gene from strains of typical and atypical enteropathogenic Escherichia coli (EPEC) and Verocytotoxin-producing E. coli (VTEC) were compared. A novel HhaI PCR-RFLP method was developed that was rapid, easy to use and amplified an 1852 bp fragment of the intimin gene from all isolates examined. This method was used to investigate the intimin sub-types of EPEC strains associated with 14 outbreaks of diarrhoeal disease between 1967 and 2001, and 20 sporadic cases between January and December 2000, in the UK and Eire. In this study, genes encoding α, β, γ, δ and ζ-intimin were detected in the EPEC strains associated with outbreaks and β, γ, ε, θ and ζ-intimin genes were identified in isolates from sporadic cases. The β-intimin gene was the most frequently detected sub-type in both the outbreak and sporadic strains.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalMolecular and Cellular Probes
Issue number4
Publication statusPublished - 1 Jan 2003
Externally publishedYes


  • Enteropathogenic Escherichia coli
  • Intimin typing
  • Verocytotoxigenic Escherichia coli

Cite this