Substrate specificity of a mouse aldo-keto reductase (AKR1C12)

Satoshi Endo, Kengo Matsumoto, Toshiyuki Matsunaga, Shuhei Ishikura, Kazuo Tajima, Ossama El-Kabbani, Akira Hara

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9 Citations (Scopus)

Abstract

AKR1C12, a mouse member of the aldo-keto reductase (AKR) superfamily, is highly expressed in the stomach and is identical to a protein encoded in an interleukin-3-regulated gene in mouse myeloid cells, but its function remains unknown. In this study, the recombinant AKR1C12 was purified to homogeneity and the specificity for coenzymes and substrates was examined at a physiological pH of 7.4. The enzyme reduced various a-dicarbonyl compounds, several ketosteroids, aldchydes and some ketones using NADH as the preferred coenzyme. In the reverse reaction, the enzyme showed coenzyme preference for NAD(+), and oxidized 3 alpha-, 17 beta- and 20 alpha-hydroxysteroids, and non-steroidal aliphatic and alicyclic alcohols, of which many hydroxysteroids and geranylgeraniol were good substrates, exhibiting low K-m and high k(cat)/K-m values. The results, together with the intracellular high ratio of NAD(+)/NADH, suggest that AKR1C12 functions as a dehydrogenase for the endogenous hydroxysteroids and geranylgeraniol in mouse stomach and myeloid cells.
Original languageEnglish
Pages (from-to)2488 - 2492
Number of pages5
JournalBiological & Pharmaceutical Bulletin
Volume29
Issue number12
Publication statusPublished - 2006

Cite this

Endo, S., Matsumoto, K., Matsunaga, T., Ishikura, S., Tajima, K., El-Kabbani, O., & Hara, A. (2006). Substrate specificity of a mouse aldo-keto reductase (AKR1C12). Biological & Pharmaceutical Bulletin, 29(12), 2488 - 2492.
Endo, Satoshi ; Matsumoto, Kengo ; Matsunaga, Toshiyuki ; Ishikura, Shuhei ; Tajima, Kazuo ; El-Kabbani, Ossama ; Hara, Akira. / Substrate specificity of a mouse aldo-keto reductase (AKR1C12). In: Biological & Pharmaceutical Bulletin. 2006 ; Vol. 29, No. 12. pp. 2488 - 2492.
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Endo, S, Matsumoto, K, Matsunaga, T, Ishikura, S, Tajima, K, El-Kabbani, O & Hara, A 2006, 'Substrate specificity of a mouse aldo-keto reductase (AKR1C12)', Biological & Pharmaceutical Bulletin, vol. 29, no. 12, pp. 2488 - 2492.

Substrate specificity of a mouse aldo-keto reductase (AKR1C12). / Endo, Satoshi; Matsumoto, Kengo; Matsunaga, Toshiyuki; Ishikura, Shuhei; Tajima, Kazuo; El-Kabbani, Ossama; Hara, Akira.

In: Biological & Pharmaceutical Bulletin, Vol. 29, No. 12, 2006, p. 2488 - 2492.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Endo, Satoshi

AU - Matsumoto, Kengo

AU - Matsunaga, Toshiyuki

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AU - Tajima, Kazuo

AU - El-Kabbani, Ossama

AU - Hara, Akira

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Endo S, Matsumoto K, Matsunaga T, Ishikura S, Tajima K, El-Kabbani O et al. Substrate specificity of a mouse aldo-keto reductase (AKR1C12). Biological & Pharmaceutical Bulletin. 2006;29(12):2488 - 2492.