Subset analysis of human and mouse mature NK cells.

Yoshihiro Hayakawa; Daniel M. Andrews; Mark J. Smyth

Subset analysis of human and mouse mature NK cells.

Yoshihiro Hayakawa, Daniel M. Andrews, Mark J. Smyth

Immunology Alfred Hospital

Research output: Contribution to journal › Article › Research › peer-review

22 Citations (Scopus)

Abstract

Identification of natural killer (NK) cell subsets has gained attention with the recent discovery that mature mouse NK cells comprise two distinct stages. Delineation of the stages is performed using the markers CD27 and CD11b on gated NK cells. The significance of this finding is underpinned by recent discoveries that mature human NK cells can also be discriminated by differential expression of CD27. This chapter will describe the methods required for the purification of lymphocytes from blood and other organs and the delineation of NK cell subsets by flow cytometry.

Original language	English
Pages (from-to)	27-38
Number of pages	12
Journal	Methods in Molecular Biology
Volume	612
Publication status	Published - 2010

Cite this

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abstract = "Identification of natural killer (NK) cell subsets has gained attention with the recent discovery that mature mouse NK cells comprise two distinct stages. Delineation of the stages is performed using the markers CD27 and CD11b on gated NK cells. The significance of this finding is underpinned by recent discoveries that mature human NK cells can also be discriminated by differential expression of CD27. This chapter will describe the methods required for the purification of lymphocytes from blood and other organs and the delineation of NK cell subsets by flow cytometry.",

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AB - Identification of natural killer (NK) cell subsets has gained attention with the recent discovery that mature mouse NK cells comprise two distinct stages. Delineation of the stages is performed using the markers CD27 and CD11b on gated NK cells. The significance of this finding is underpinned by recent discoveries that mature human NK cells can also be discriminated by differential expression of CD27. This chapter will describe the methods required for the purification of lymphocytes from blood and other organs and the delineation of NK cell subsets by flow cytometry.

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Subset analysis of human and mouse mature NK cells.

Abstract

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