TY - JOUR
T1 - Subcutaneous injection of pluripotent stem cells in mice
AU - Nagy, Kristina Vintersten
AU - Nagy, Andras
PY - 2018/4/1
Y1 - 2018/4/1
N2 - This protocol describes the production of teratomas and teratocarcinomas used to determine the in vivo developmental potential of adult or embryonic tissues, including early-stage embryos, somites, and tail bud, as well as embryonic and other pluripotent stem cells. Resulting tumors are screened by histology and markers to assess differentiation of the tissues. Methods and sites may vary, depending on cell and tissue type. Subcutaneous injections are easy to perform and do not need surgical manipulations. Suspension of cells in Matrigel Basement Membrane (BD Biosciences) promotes their anatomical localization and is a commonly used, efficient, and reproducible approach.
AB - This protocol describes the production of teratomas and teratocarcinomas used to determine the in vivo developmental potential of adult or embryonic tissues, including early-stage embryos, somites, and tail bud, as well as embryonic and other pluripotent stem cells. Resulting tumors are screened by histology and markers to assess differentiation of the tissues. Methods and sites may vary, depending on cell and tissue type. Subcutaneous injections are easy to perform and do not need surgical manipulations. Suspension of cells in Matrigel Basement Membrane (BD Biosciences) promotes their anatomical localization and is a commonly used, efficient, and reproducible approach.
UR - http://www.scopus.com/inward/record.url?scp=85044917897&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot094078
DO - 10.1101/pdb.prot094078
M3 - Article
C2 - 29610361
AN - SCOPUS:85044917897
SN - 1940-3402
VL - 2018
SP - 251
EP - 252
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 4
ER -