Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

The cells of the adaptive immune system, B and T lymphocytes, each generate a unique antigen receptor through V(D)J recombination of their immunoglobulin (Ig) and T-cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot replicate and have no function in the cell. Since the coding joint in the genome is replicated with each cell division, the ratio between coding joints and signal joints in a population of B cells can be used as a measure for proliferation. This chapter describes a real-time quantitative (RQ-)PCR-based approach to quantify proliferation through calculating the ratio between coding joints and signal joints of the frequently occurring intronRSS–Kde rearrangements in the IGK light chain locus. Besides its use in normal B-cell biology, quantification of B-cell replication can inform on abnormal proliferation in human diseases and in B-cell neogenesis following depletion therapy.

Original languageEnglish
Title of host publicationLymphoma
Subtitle of host publicationMethods and Protocols
EditorsRalf Küppers
Place of PublicationNew York, NY
PublisherHumana Press
Chapter6
Pages127-138
Number of pages12
Edition2nd
ISBN (Electronic)9781493991518
ISBN (Print)9781493991501
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1956
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • B cell
  • IGK
  • Immunoglobulin
  • IntronRSS
  • Kde
  • KREC
  • Proliferation
  • Real-time quantitative PCR
  • Replication history
  • V(D)J recombination

Cite this

van Zelm, M. C. (2019). Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR. In R. Küppers (Ed.), Lymphoma: Methods and Protocols (2nd ed., pp. 127-138). (Methods in Molecular Biology; Vol. 1956). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9151-8_6
van Zelm, Menno C. / Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR. Lymphoma: Methods and Protocols. editor / Ralf Küppers. 2nd . ed. New York, NY : Humana Press, 2019. pp. 127-138 (Methods in Molecular Biology).
@inbook{26805774f0704d8688e6959d0dfc0d0d,
title = "Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR",
abstract = "The cells of the adaptive immune system, B and T lymphocytes, each generate a unique antigen receptor through V(D)J recombination of their immunoglobulin (Ig) and T-cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot replicate and have no function in the cell. Since the coding joint in the genome is replicated with each cell division, the ratio between coding joints and signal joints in a population of B cells can be used as a measure for proliferation. This chapter describes a real-time quantitative (RQ-)PCR-based approach to quantify proliferation through calculating the ratio between coding joints and signal joints of the frequently occurring intronRSS–Kde rearrangements in the IGK light chain locus. Besides its use in normal B-cell biology, quantification of B-cell replication can inform on abnormal proliferation in human diseases and in B-cell neogenesis following depletion therapy.",
keywords = "B cell, IGK, Immunoglobulin, IntronRSS, Kde, KREC, Proliferation, Real-time quantitative PCR, Replication history, V(D)J recombination",
author = "{van Zelm}, {Menno C.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9151-8_6",
language = "English",
isbn = "9781493991501",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "127--138",
editor = "K{\"u}ppers, {Ralf }",
booktitle = "Lymphoma",
address = "United States of America",
edition = "2nd",

}

van Zelm, MC 2019, Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR. in R Küppers (ed.), Lymphoma: Methods and Protocols. 2nd edn, Methods in Molecular Biology, vol. 1956, Humana Press, New York, NY, pp. 127-138. https://doi.org/10.1007/978-1-4939-9151-8_6

Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR. / van Zelm, Menno C.

Lymphoma: Methods and Protocols. ed. / Ralf Küppers. 2nd . ed. New York, NY : Humana Press, 2019. p. 127-138 (Methods in Molecular Biology; Vol. 1956).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

TY - CHAP

T1 - Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR

AU - van Zelm, Menno C.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The cells of the adaptive immune system, B and T lymphocytes, each generate a unique antigen receptor through V(D)J recombination of their immunoglobulin (Ig) and T-cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot replicate and have no function in the cell. Since the coding joint in the genome is replicated with each cell division, the ratio between coding joints and signal joints in a population of B cells can be used as a measure for proliferation. This chapter describes a real-time quantitative (RQ-)PCR-based approach to quantify proliferation through calculating the ratio between coding joints and signal joints of the frequently occurring intronRSS–Kde rearrangements in the IGK light chain locus. Besides its use in normal B-cell biology, quantification of B-cell replication can inform on abnormal proliferation in human diseases and in B-cell neogenesis following depletion therapy.

AB - The cells of the adaptive immune system, B and T lymphocytes, each generate a unique antigen receptor through V(D)J recombination of their immunoglobulin (Ig) and T-cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot replicate and have no function in the cell. Since the coding joint in the genome is replicated with each cell division, the ratio between coding joints and signal joints in a population of B cells can be used as a measure for proliferation. This chapter describes a real-time quantitative (RQ-)PCR-based approach to quantify proliferation through calculating the ratio between coding joints and signal joints of the frequently occurring intronRSS–Kde rearrangements in the IGK light chain locus. Besides its use in normal B-cell biology, quantification of B-cell replication can inform on abnormal proliferation in human diseases and in B-cell neogenesis following depletion therapy.

KW - B cell

KW - IGK

KW - Immunoglobulin

KW - IntronRSS

KW - Kde

KW - KREC

KW - Proliferation

KW - Real-time quantitative PCR

KW - Replication history

KW - V(D)J recombination

UR - http://www.scopus.com/inward/record.url?scp=85061846057&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9151-8_6

DO - 10.1007/978-1-4939-9151-8_6

M3 - Chapter (Book)

SN - 9781493991501

T3 - Methods in Molecular Biology

SP - 127

EP - 138

BT - Lymphoma

A2 - Küppers, Ralf

PB - Humana Press

CY - New York, NY

ER -

van Zelm MC. Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR. In Küppers R, editor, Lymphoma: Methods and Protocols. 2nd ed. New York, NY: Humana Press. 2019. p. 127-138. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9151-8_6