Research output per year
Research output per year
Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review
The cells of the adaptive immune system, B and T lymphocytes, each generate a unique antigen receptor through V(D)J recombination of their immunoglobulin (Ig) and T-cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot replicate and have no function in the cell. Since the coding joint in the genome is replicated with each cell division, the ratio between coding joints and signal joints in a population of B cells can be used as a measure for proliferation. This chapter describes a real-time quantitative (RQ-)PCR-based approach to quantify proliferation through calculating the ratio between coding joints and signal joints of the frequently occurring intronRSS–Kde rearrangements in the IGK light chain locus. Besides its use in normal B-cell biology, quantification of B-cell replication can inform on abnormal proliferation in human diseases and in B-cell neogenesis following depletion therapy.
Original language | English |
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Title of host publication | Lymphoma |
Subtitle of host publication | Methods and Protocols |
Editors | Ralf Küppers |
Place of Publication | New York, NY |
Publisher | Humana Press |
Chapter | 6 |
Pages | 127-138 |
Number of pages | 12 |
Edition | 2nd |
ISBN (Electronic) | 9781493991518 |
ISBN (Print) | 9781493991501 |
DOIs | |
Publication status | Published - 1 Jan 2019 |
Name | Methods in Molecular Biology |
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Publisher | Humana Press |
Volume | 1956 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review