Studies of the secretion of corticotropin-releasing factor and arginine vasopressin into the hypophysial-portal circulation of the conscious sheep: I. effect of an audiovisual stimulus and insulin-induced hypoglycemia

Dennis Engler, Thao Pham, Meryl J. Fullerton, Guck Ooi, John W. Funder, Iain J. Clarke

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Abstract

Studies were undertaken to characterize the secretion of corticotropin-releasing factor (CRF) and arginine vasopressin (AVP) into the hypophysial-portal circulation ofthe conscious sheep. In addition, we examined the temporal relationship between the secretion of these two hypothalamic peptides and the secretion of three proopiomelanocortin peptides - adrenocorticotropic hormone (ACTH), ir-β-endorphin, and ir-αmelanocyte-stimtilaiing hormone - and Cortisol and determined the effects of an audiovisual emotional stimulus and insulin-induced hypoglycemia on the entire hypo-thalamic-pituitary-adrenal axis. In the basal state, the secretion of CRF. AVP, the three proopiomelanocortin peptides, and Cortisol was pulsatile in nature, and three CRF and AVP pulse patterns were observed: a concordant increase in CRF and AVP, an isolated rise in CRF, and an isolated increase in AVP. In 4 of the 5 animals, a 3-mtn audiovisual stress (barking dog) rapidly increased the plasma levels of all the measured substances, although the magnitude and duration of the effect differed markedly between the animals. Insulin-induced hypoglycemia markedly increased AVP and, to a lesser extent, CRF concentrations in portal plasma and thereby altered the CRF:AVP molar ratio. Although pituitary-adrenal activation was closely correlated with the increased hypothalamic activity, a strict 1:1 concordance between CRF/AVP secretion and ACTH secretion was not seen. The anesthetic ketamine selectively increased portal AVP concentrations to levels which exceeded those attained during hypoglycemia and rapidly activated the pituitary-adrenal axis. We conclude the following: (1) CRF and AVP are secreted by the hypothalamus in a pulsatile fashion; (2) ACTH secretion can be stimulated by increases in cither CRF or AVP; (3) the absence of a strict I: I concordance between hypothalamic CRF/AVP release and pituitary ACTH secretion during stress may be partly due to the release of additional hypothalamic ACTH secretagogues; (4) the ability of both audiovisual stimuli and tnsulín-índuced hypoglycemia to augment CRF and AVP secretion indicates that the paraventricular hypothalamus may be activated by a variety of neural inputs, and (5) the marked alteration of the CRF:AVP molar ratio during stress suggests that AVP may be an important ACTH secretagogue in vivo in the sheep.

Original languageEnglish
Pages (from-to)367-381
Number of pages15
JournalNeuroendocrinology
Volume49
Issue number4
DOIs
Publication statusPublished - 1 Jan 1989
Externally publishedYes

Keywords

  • Arginine vasopressin
  • Audiovisual stress
  • Corticotropin-releasing factor
  • Cortisol
  • Hypoglycemia
  • Proopiomelanocortin peptides
  • Pulsatile secretion

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