Structures of thermolabile mutants of human glutathione transferase P1-1

Jamie Rossjohn, William J. McKinstry, Aaron J. Oakley, Michael W. Parker, Gun Stenberg, Bengt Mannervik, Beatrice Dragani, Roberta Cocco, Antonio Aceto

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)


An N-capping box motif (Ser/Thr-Xaa-Xaa-Asp) is strictly conserved at the beginning of helix α6 in the core of virtually all glutathione transferases (GST) and GST-related proteins. It has been demonstrated that this local motif is important in determining the α-helical propensity of the isolated α6-peptide and plays a crucial role in the folding and stability of GSTs. Its removal by site-directed mutagenesis generated temperature-sensitive folding mutants unable to refold at physiological temperature (37°C). In the present work, variants of human GSTP1-1 (S150A and D153A), in which the capping residues have been substituted by alanine, have been generated and purified for structural analysis. Thus, for the first time, temperature-sensitive folding mutants of an enzyme, expressed at a permissive temperature, have been crystallized and their three-dimensional structures determined by X-ray crystallography. The crystal structures of human pi class GST temperature-sensitive mutants provide a basis for understanding the structural origin of the dramatic effects observed on the overall stability of the enzyme at higher temperatures upon single substitution of a capping residue. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)295-302
Number of pages8
JournalJournal of Molecular Biology
Issue number2
Publication statusPublished - 15 Sep 2000
Externally publishedYes


  • Glutathione transferase
  • Helix capping
  • Mutations
  • Protein folding
  • X-ray crystallography

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