Structures of Human Peroxiredoxin 3 Suggest Self-Chaperoning Assembly that Maintains Catalytic State

N.  Amy Yewdall, Hariprasad Venugopal, Ambroise Desfosses, Vahid Abrishami, Yuliana Yosaatmadja, Mark B B. Hampton, Juliet A A. Gerrard, David C C. Goldstone, Alok K Mitra, Mazdak Radjainia

Research output: Contribution to journalArticleResearchpeer-review

25 Citations (Scopus)

Abstract

Peroxiredoxins are antioxidant proteins primarily responsible for detoxification of hydroperoxides in cells. On exposure to various cellular stresses, peroxiredoxins can acquire chaperone activity, manifested as quaternary reorganization into a high molecular weight (HMW) form. Acidification, for example, causes dodecameric rings of human peroxiredoxin 3 (HsPrx3) to stack into long helical filaments. In this work, a 4.1-Å resolution structure of low-pH-instigated helical filaments was elucidated, showing a locally unfolded active site and partially folded C terminus. A 2.8-Å crystal structure of HsPrx3 was determined at pH 8.5 under reducing conditions, wherein dodecameric rings are arranged as a short stack, with symmetry similar to low-pH filaments. In contrast to previous observations, the crystal structure displays both a fully folded active site and ordered C terminus, suggesting that the HsPrx3 HMW form maintains catalytic activity. We propose a new role for the HMW form as a self-chaperoning assembly maintaining HsPrx3 function under stress.

Original languageEnglish
Pages (from-to)1120-1129
Number of pages10
JournalStructure
Volume24
Issue number7
DOIs
Publication statusPublished - 6 Jul 2016

Keywords

  • cryo-electron microscopy
  • high molecular weight form
  • molecular chaperone
  • peroxiredoxin
  • X-Ray crystal structure

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